Quantitative electrochemiluminescence detection of proteins: avidin-based sensor and tris(2,2 '-bipyridine) ruthenium(ii) label
文献类型：期刊论文
| 作者 | Fang, Lanyun; Lue, Zhaozi; Wei, Hui; Wang, Erkang |
| 刊名 | Biosensors & bioelectronics
 |
| 出版日期 | 2008-06-15 |
| 卷号 | 23期号:11页码:1645-1651 |
| 关键词 | Quantitative electrochemiluminescence Avidin-based sensor Tris(2,2 '-bipyridine) ruthenium(ii) label Multi-wall carbon nanotube Protein |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2008.01.023 |
| 通讯作者 | Wang, erkang(ekwang@ciac.jl.cn) |
| 英文摘要 | Quantitative electrochemilumineseence (ecl) detection of a model protein, bovine serum albumin (bsa) was achieved via biotin-avidin interaction using an avidin-based sensor and a well-developed ecl system of tris(2,2'-bipyridine) ruthenium(ii) derivative as label and tri-n-propylamine (tpa) as coreactant. to detect the protein, avidin was linked to the glassy carbon electrode through passive adsorptions and covalent interaction with carboxylate-terminated carbon nanotubes that was used as binder to immobilize avidin onto the electrode. then, biotinylated bsa tagged with tris(2,2'-bipyridine) ruthenium(ii) label was attached to the prepared avidin surface. after binding of bsa labeled with tris(2,2'-bipyridine) ruthenium(ii) derivative to the surface-immobilized avidin through biotin, ecl response was generated when the self-assembled modified electrode was immersed in a tpa-containing electrolyte solution. such double protein labeling protocol with a biotin label for biorecognition and ruthenium label for ecl detection facilitated the detection of protein compared to the classical double antibody sandwich format. the ecl intensity was linearly proportional to the feed concentration of bsa over two orders of magnitude in the range of 15 nm to 7.5 mu m. the detection limit was estimated to be 1.5 nm. further application to the lysozyme analysis was carried out to validate the present approach for an effective and favorable protocol for the quantitative detection of proteins. the dynamic range of lysozyme was from 0.001 g l-1 to 0.1 g l-1 and the detection limit was 0.1 mg l-1. electrochemical impedance and cyclic voltammetric measurements along with some necessary control experiments were conducted to characterize the successful formation of self-assembled modified electrodes and to grant the whole detection process. (c) 2008 elsevier b.v. all rights reserved. |
| WOS关键词 | C-REACTIVE PROTEIN ; ELECTROGENERATED CHEMILUMINESCENCE ; CARBON NANOTUBES ; CAPILLARY-ELECTROPHORESIS ; IMPEDANCE SPECTROSCOPY ; HIGH AMPLIFICATION ; ELECTRODES ; DNA ; ASSEMBLIES ; COMPLEXES |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| WOS记录号 | WOS:000256491800010 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2387374 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Erkang |
| 作者单位 | Chinese Acad Sci, Grad Sch, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Lanyun,Lue, Zhaozi,Wei, Hui,et al. Quantitative electrochemiluminescence detection of proteins: avidin-based sensor and tris(2,2 '-bipyridine) ruthenium(ii) label[J]. Biosensors & bioelectronics,2008,23(11):1645-1651. |
| APA | Fang, Lanyun,Lue, Zhaozi,Wei, Hui,&Wang, Erkang.(2008).Quantitative electrochemiluminescence detection of proteins: avidin-based sensor and tris(2,2 '-bipyridine) ruthenium(ii) label.Biosensors & bioelectronics,23(11),1645-1651. |
| MLA | Fang, Lanyun,et al."Quantitative electrochemiluminescence detection of proteins: avidin-based sensor and tris(2,2 '-bipyridine) ruthenium(ii) label".Biosensors & bioelectronics 23.11(2008):1645-1651. |
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来源：中国科学院大学
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