Molecular cloning, functional characterization and expression analysis of a novel monosaccharide transporter gene osmst6 from rice (oryza sativa l.)
文献类型：期刊论文
| 作者 | Wang, Yongqin1,2,5; Xiao, Yuguo1,2,3,5; Zhang, Yu1,2,3,5; Chai, Chenglin1,2,5; Wei, Gang1,2,5; Wei, Xiaoli1,2; Xu, Honglin1,2; Wang, Mei3,4; Ouwerkerk, Pieter B. F.3; Zhu, Zhen1,2 |
| 刊名 | Planta
 |
| 出版日期 | 2008-09-01 |
| 卷号 | 228期号:4页码:525-535 |
| 关键词 | Cdna cloning Expression pattern Monosaccharide transporter Oryza Seed development |
| ISSN号 | 0032-0935 |
| DOI | 10.1007/s00425-008-0755-8 |
| 通讯作者 | Zhu, zhen(zzhu@genetics.ac.cn) |
| 英文摘要 | Monosaccharides transporters play important roles in assimilate supply for sink tissue development. in this study, a new monosaccharide transporter gene osmst6 was identified from rice (oryza sativa l.). the predicted osmst6 protein shows typical features of sugar transporters and shares 79.6% identity with the rice monosaccharide transporter osmst3. heterologous expression in yeast (saccharomyces cerevisiae) demonstrated that osmst6 is a broad-spectrum monosaccharide transporter, with a km of 266.1 mu m for glucose. osmst6-green fluorescent protein fusion protein is localized to the plasma membrane in plant. semi-quantitative rt-pcr analysis exhibited that osmst6 is expressed in all tested organs/tissues. in developing seeds, osmst6 expression level is high at the early and middle grain filling stages and gradually declines later. further analysis detected its expression in both maternal and filial tissues. rna in situ hybridization analysis indicated that osmst6 is predominantly expressed in the vascular parenchyma of the chalazal vein, cross-cells, nucellar tissue and endosperm of young seeds, in mesophyll cells of source leaf blades, and in pollens and the connective vein of anthers. in addition, osmst6 expression is up-regulated by salt stress and sugars. the physiological role of osmst6 for seed development and its roles in other sink and source tissues are discussed. |
| WOS关键词 | CELL-WALL INVERTASE ; HEXOSE TRANSPORTERS ; SUCROSE TRANSPORTER ; SEQUENCE ALIGNMENT ; GENOMIC DNA ; ARABIDOPSIS ; FAMILY ; PLANTS ; GRAIN ; CARYOPSIS |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000259008800001 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2387630 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhu, Zhen |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 3.Leiden Univ, Dept Mol & Dev Genet, Inst Biol IBL, NL-2300 RA Leiden, Netherlands 4.TNO Qual Life, NL-2333 CK Leiden, Netherlands 5.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yongqin,Xiao, Yuguo,Zhang, Yu,et al. Molecular cloning, functional characterization and expression analysis of a novel monosaccharide transporter gene osmst6 from rice (oryza sativa l.)[J]. Planta,2008,228(4):525-535. |
| APA | Wang, Yongqin.,Xiao, Yuguo.,Zhang, Yu.,Chai, Chenglin.,Wei, Gang.,...&Zhu, Zhen.(2008).Molecular cloning, functional characterization and expression analysis of a novel monosaccharide transporter gene osmst6 from rice (oryza sativa l.).Planta,228(4),525-535. |
| MLA | Wang, Yongqin,et al."Molecular cloning, functional characterization and expression analysis of a novel monosaccharide transporter gene osmst6 from rice (oryza sativa l.)".Planta 228.4(2008):525-535. |
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来源：中国科学院大学
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