Inhibition of protein tyrosine phosphatase 1b and alkaline phosphatase by bis(maltolato)oxovanadium (iv)
文献类型：期刊论文
| 作者 | Li, Ming1; Ding, Wenjun1; Baruah, Bharat2; Crans, Debbie C.2; Wang, Ruilin1 |
| 刊名 | Journal of inorganic biochemistry
 |
| 出版日期 | 2008-10-01 |
| 卷号 | 102期号:10页码:1846-1853 |
| 关键词 | Inhibition Bis(maltolato)oxovanadium(iv) Glutathione s-transferase-tagged protein Tyrosine phosphatase 1b Alkaline phosphatase |
| ISSN号 | 0162-0134 |
| DOI | 10.1016/j.jinorgbio.2008.06.007 |
| 通讯作者 | Ding, wenjun(dingwj@gucas.ac.cn) |
| 英文摘要 | Vanadate has been recognized as a specific and potent phosphatase inhibitor since its structure is similar to that of phosphate. in this study, we measured the inhibition of glutathione s-transferase-tagged protein tyrosine phosphatase 1b (gst-ptp1b) and alkaline phosphatase (alp) by the insulin enhancing compounds, bis(maltolato)oxovanadium(iv) (bmov). the results showed that the activity of gst-ptp1b was reversibly inhibited by solutions of bmov with an ic(50) value of 0.86 +/- 0.02 mu m. steady state kinetic studies showed that inhibition of gst-ptp1b by bmov was of a mixed competitive and noncompetitive type. in addition, incubation of gst-ptp1b with bmov showed a time-dependent biphasic inactivation of the protein. on the other hand, the inhibitory behavior of bmov on alp activity was reversible and competitive with an ic(50) value of 32.1 +/- 0.6 mu m. incubation with bmov did not show biphasic inactivation of alp. the reversible inhibition of gst-ptp1b by bmov is more potent than that of alp, but solutions of bmov inhibited both enzymes. this data support the suggestion that mechanisms for the inhibitory effects of bmov on gst-ptp1b and alp are very different. (c) 2008 published by elsevier inc. |
| WOS关键词 | PROTEIN-TYROSINE PHOSPHATASES ; INSULIN SENSITIVITY ; VANADATE TREATMENT ; VANADIUM COMPOUNDS ; DIABETIC-RATS ; IN-VIVO ; REDUCTION ; MECHANISM ; GLUCOSE ; MICE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Inorganic & Nuclear |
| 语种 | 英语 |
| WOS记录号 | WOS:000259385700003 |
| 出版者 | ELSEVIER SCIENCE INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2387729 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ding, Wenjun |
| 作者单位 | 1.Chinese Acad Sci, Grad Univ, Coll Life Sci, Beijing 100049, Peoples R China 2.Colorado State Univ, Dept Chem, Ft Collins, CO 80523 USA |
| 推荐引用方式 GB/T 7714 | Li, Ming,Ding, Wenjun,Baruah, Bharat,et al. Inhibition of protein tyrosine phosphatase 1b and alkaline phosphatase by bis(maltolato)oxovanadium (iv)[J]. Journal of inorganic biochemistry,2008,102(10):1846-1853. |
| APA | Li, Ming,Ding, Wenjun,Baruah, Bharat,Crans, Debbie C.,&Wang, Ruilin.(2008).Inhibition of protein tyrosine phosphatase 1b and alkaline phosphatase by bis(maltolato)oxovanadium (iv).Journal of inorganic biochemistry,102(10),1846-1853. |
| MLA | Li, Ming,et al."Inhibition of protein tyrosine phosphatase 1b and alkaline phosphatase by bis(maltolato)oxovanadium (iv)".Journal of inorganic biochemistry 102.10(2008):1846-1853. |
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来源：中国科学院大学
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