Reversed-Phase-Reversed-Phase Liquid Chromatography Approach with High Orthogonality for Multidimensional Separation of Phosphopeptides
文献类型：期刊论文
| 作者 | Song, Chunxia1,2,3; Ye, Mingliang1,2; Han, Guanghui1,2,3; Jiang, Xinning1,2,3; Wang, Fangjun1,2,3; Yu, Zhiyuan1,2,3; Chen, Rui1,2,3; Zou, Hanfa1,2 |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2010 |
| 卷号 | 82期号:1页码:53-56 |
| ISSN号 | 10.1021/ac9023044 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | protein phosphorylation regulates a series of important biological processes in eukaryotes. however, the phosphorylation sites found up to now are far below than that actually exists in proteins due to the extreme complexity of the proteome sample. here a new reversed-phase-reversed-phase liquid chromatography (rp-rplc) approach was developed for multidimensional separation of phosphopeptides. in this approach, a large number of fractions were collected from the first dimensional rplc separation at high ph. and then these fractions were pooled every two fractions with equal time interval, one from the early eluted section and another one from the, later eluted section. the pooled fractions were finally submitted to rplc-tandem mass spectrometry (ms/ms) analysis at low ph. it was found the resulting 2d separation was highly orthogonal and yielded more than 30% phosphopeptide identifications over the conventional rplc,c approach. this study provides a powerful approach for efficient separation of phosphopeptides and global phosphorylation analysis, where the orthogonality of 2d separation is greatly improved and the first dimensional separation is of high resolution. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | phosphoproteome analysis ; phosphorylation analysis ; mass-spectrometry ; resolution ; hplc |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000273265700014 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/103053]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Chunxia,Ye, Mingliang,Han, Guanghui,et al. Reversed-Phase-Reversed-Phase Liquid Chromatography Approach with High Orthogonality for Multidimensional Separation of Phosphopeptides[J]. analytical chemistry,2010,82(1):53-56. |
| APA | Song, Chunxia.,Ye, Mingliang.,Han, Guanghui.,Jiang, Xinning.,Wang, Fangjun.,...&Zou, Hanfa.(2010).Reversed-Phase-Reversed-Phase Liquid Chromatography Approach with High Orthogonality for Multidimensional Separation of Phosphopeptides.analytical chemistry,82(1),53-56. |
| MLA | Song, Chunxia,et al."Reversed-Phase-Reversed-Phase Liquid Chromatography Approach with High Orthogonality for Multidimensional Separation of Phosphopeptides".analytical chemistry 82.1(2010):53-56. |
入库方式： OAI收割
来源：大连化学物理研究所
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