Two domains of the epidermal growth factor receptor are involved in cytoskeletal interactions
文献类型：期刊论文
| 作者 | Song, Wei1,2; Wu, Jing1,2; Ge, Gaoxiang1,2; Lin, Qishui1 |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2008-06-13 |
| 卷号 | 370期号:4页码:589-593 |
| 关键词 | Egfr Actin Fret Actin-binding domain Yeast two-hybrid system |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2008.03.138 |
| 通讯作者 | Lin, qishui(qslin@sibs.ac.cn) |
| 英文摘要 | Epidermal growth factor receptor can interact directly with f-actin through an actin-binding domain. in the present study, a mutant egfr, lacking a previously identified actin-binding domain (abd 1), was still able to bind elements of the cytoskeleton. a second egfr actin-binding domain (abd 2) was identified in the region of the receptor that includes tyr-1 148 by a yeast two-hybrid assay. gst fusion proteins comprising abd 1 or abd 2 bound actin in vitro and competed for actin-binding with the full-length egfr. egfr binding to actin was also studied in intact cells using fluorescence resonance energy transfer (fret). the localization of the egfr/actin-binding complex changed after egf stimulation. fusion proteins containing mutations in abid1 or abd2 did not display a fret signal. the results lead to the conclusion that the interaction between abd1 and abd2 and actin during egf-induced signal transduction, and thus between egfr and actin, are important in cell activation. (c) 2008 elsevier inc. all rights reserved. |
| WOS关键词 | ACTIN-BINDING DOMAIN ; CARCINOMA A431 CELLS ; EGF RECEPTOR ; LIGAND-BINDING ; PHOSPHORYLATION ; ASSOCIATION ; ACTIVATION ; A431-CELLS ; AFFINITY ; PROTEIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000255883900012 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2387782 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Lin, Qishui |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Mol Cell Biol, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Wei,Wu, Jing,Ge, Gaoxiang,et al. Two domains of the epidermal growth factor receptor are involved in cytoskeletal interactions[J]. Biochemical and biophysical research communications,2008,370(4):589-593. |
| APA | Song, Wei,Wu, Jing,Ge, Gaoxiang,&Lin, Qishui.(2008).Two domains of the epidermal growth factor receptor are involved in cytoskeletal interactions.Biochemical and biophysical research communications,370(4),589-593. |
| MLA | Song, Wei,et al."Two domains of the epidermal growth factor receptor are involved in cytoskeletal interactions".Biochemical and biophysical research communications 370.4(2008):589-593. |
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来源：中国科学院大学
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