Integrative proteomic and cytological analysis of the effects of extracellular ca(2+) influx on pinus bungeana pollen tube development
文献类型：期刊论文
| 作者 | Wu, Xiaoqin1,2; Chen, Tong1,3; Zheng, Maozhong1,3; Chen, Yanmei1; Teng, Nianjun1,3; Samaj, Jozef4,5; Baluska, Frantisek4,6; Lin, Jinxing1 |
| 刊名 | Journal of proteome research
 |
| 出版日期 | 2008-10-01 |
| 卷号 | 7期号:10页码:4299-4312 |
| 关键词 | Calcium Nifedipine Pinus bungeana Proteomics Pollen tube |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/pr800241u |
| 通讯作者 | Lin, jinxing(linjx@ibcas.ac.cn) |
| 英文摘要 | Ca(2+) is an essential ion in the control of pollen germination and tube growth. however, the control of pollen tube development by ca(2+) signaling and its interactions with cytoskeletal components, energy-providing pathways, and cell-expansion machinery remain elusive. here, we used nifedipine (nif) to study ca(2+) functions in differential protein expression and other cellular processes in pinus bungeana pollen tube growth. proteomics analysis indicated that 50 proteins showed differential expression with varying doses of nif. thirty-four of these were homologous to previously reported proteins and were classified into different functional categories closely related to tip-growth machinery. blocking the l-type ca(2+) channel with nif in the pollen tube membrane induced several early alterations within a short time, including a reduction of extracellular ca(2+) influx and a subsequently dramatic decrease in cytosolic free ca(2+) concentration ([ca(2+)]), concomitant with ultrastructural abnormalities and changes in the abundance of proteins involved in energy production and signaling. secondary alterations included actin filament depolymerization, disrupted patterns of endocytosis/exocytosis, and cell wall remodeling, along with changes in the proteins involved in these processes. these results suggested that extracellular ca(2+) ` influx was necessary for the maintenance of the typical tip-focused [ca(2+)] gradient in the p. bungeana pollen tube, and that reduced adenosine triphosphate production (atp), depolymerization of the cytoskeleton, and abnormal endocytosis/exocytosis, together with enhanced rigidity of cell walls, were responsible for the growth arrest observed in pollen tubes treated with nif. |
| WOS关键词 | SELF-INCOMPATIBILITY RESPONSE ; LILY POLLEN ; ACTIN CYTOSKELETON ; LATRUNCULIN-B ; CELL-WALL ; PROTEIN-SYNTHESIS ; PAPAVER-RHOEAS ; CALCIUM INFLUX ; ATP DEPLETION ; TIP GROWTH |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemical Research Methods |
| 语种 | 英语 |
| WOS记录号 | WOS:000259784300010 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2387814 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Lin, Jinxing |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Photosynth & Mol Environm Physiol, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, S China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 4.Univ Bonn, Inst Cellular & Mol Bot, Dept Plant Cell Biol, D-53115 Bonn, Germany 5.Slovak Acad Sci, Inst Plant Genet & Biotechnol, SK-95007 Nitra, Slovakia 6.Slovak Acad Sci, Inst Bot, SK-84223 Bratislava, Slovakia |
| 推荐引用方式 GB/T 7714 | Wu, Xiaoqin,Chen, Tong,Zheng, Maozhong,et al. Integrative proteomic and cytological analysis of the effects of extracellular ca(2+) influx on pinus bungeana pollen tube development[J]. Journal of proteome research,2008,7(10):4299-4312. |
| APA | Wu, Xiaoqin.,Chen, Tong.,Zheng, Maozhong.,Chen, Yanmei.,Teng, Nianjun.,...&Lin, Jinxing.(2008).Integrative proteomic and cytological analysis of the effects of extracellular ca(2+) influx on pinus bungeana pollen tube development.Journal of proteome research,7(10),4299-4312. |
| MLA | Wu, Xiaoqin,et al."Integrative proteomic and cytological analysis of the effects of extracellular ca(2+) influx on pinus bungeana pollen tube development".Journal of proteome research 7.10(2008):4299-4312. |
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来源：中国科学院大学
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