Analysis of the Subcellular Phosphoproteome Using a Novel Phosphoproteomic Reactor
文献类型：期刊论文
| 作者 | Zhou, Houjiang1,2,3,5; Elisma, Fred2,3; Denis, Nicholas J.2,3; Wright, Theodore G.2,3; Tian, Ruijun2,3; Zhou, Hu2,3; Hou, Weimin2,4; Zou, Hanfa1,5; Figeys, Daniel2,3,4 |
| 刊名 | journal of proteome research
 |
| 出版日期 | 2010-03-01 |
| 卷号 | 9期号:3页码:1279-1288 |
| 关键词 | Phosphoproteomics proteomic reactor subcellular technology |
| ISSN号 | 1535-3893 |
| 产权排序 | 3;1 |
| 通讯作者 | 邹汉法 ; daniel figeys |
| 英文摘要 | protein phosphorylation is an important post-translational modification involved in the regulation of many cellular processes. mass spectrometry has been successfully used to identify protein phosphorylation in specific pathways and for global phosphoproteomic analysis. however, phosphoproteomics approaches do not evaluate the subcellular localization of the phosphorylated forms of proteins, which is an important factor for understanding the roles of protein phosphorylation on a global scale. the in-depth mapping of protein phosphorylation at the subcellular level necessitates the development of new methods capable of specifically and efficiently enriching phosphopeptides from highly complex samples. here, we report a novel microfluidic device called the phosphoproteomic reactor that combines efficient processing of proteins followed by phosphopeptide enrichment by ti-imac. to illustrate the potential of this novel technology, we mapped the phosphoproteins in subcellular organelles of liver cells. fifteen subcellular fractions from liver cell cultures were processed on the phosphoproteomic reactor in combination with nano-lc-ms/ms analysis. we identified thousands of phosphorylation sites in over 600 phosphoproteins in different organelles using minute amounts of starting material. overall, this approach provides a new avenue for studying the phosphoproteome of the subcellular organelles. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | ion affinity-chromatography ; highly selective enrichment ; cytoscape plug-in ; mass-spectrometry ; phosphorylated peptides ; proteomic reactor ; quantitative phosphoproteomics ; phosphopeptide enrichment ; organelle proteomics ; titanium-dioxide |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000275088100011 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/103065]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Ottawa, Ottawa Inst Syst Biol, Ottawa, ON K1H 8M5, Canada 3.Univ Ottawa, Dept Biochem Microbiol & Immunol, Fac Med, Ottawa, ON K1H 8M5, Canada 4.Univ Ottawa, Dept Chem, Ottawa, ON K1H 8M5, Canada 5.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Houjiang,Elisma, Fred,Denis, Nicholas J.,et al. Analysis of the Subcellular Phosphoproteome Using a Novel Phosphoproteomic Reactor[J]. journal of proteome research,2010,9(3):1279-1288. |
| APA | Zhou, Houjiang.,Elisma, Fred.,Denis, Nicholas J..,Wright, Theodore G..,Tian, Ruijun.,...&Figeys, Daniel.(2010).Analysis of the Subcellular Phosphoproteome Using a Novel Phosphoproteomic Reactor.journal of proteome research,9(3),1279-1288. |
| MLA | Zhou, Houjiang,et al."Analysis of the Subcellular Phosphoproteome Using a Novel Phosphoproteomic Reactor".journal of proteome research 9.3(2010):1279-1288. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。