中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Gonadotropin stimulation of ovarian fractalkine expression and fractalkine augmentation of progesterone biosynthesis by luteinizing granulosa cells

文献类型:期刊论文

作者Zhao, Ping1,3; De, Ananya2; Hu, Zeng1,3; Li, Jing2; Mulders, Sabine M.4; Gelpke, Maarten D. Sollewijn4; Duan, En-Kui1; Hsueh, Aaron J. W.2
刊名Endocrinology
出版日期2008-06-01
卷号149期号:6页码:2782-2789
ISSN号0013-7227
DOI10.1210/en.2007-1662
通讯作者Duan, en-kui(duane@ioz.ac.cn)
英文摘要Recent studies indicated that ovarian functions are regulated by diverse paracrine factors induced by the preovulatory increases in circulating lh. based on dna microarray analyses and real-time rt-pcr, we found a major increase in the transcript levels of a chemokine fractalkine after human chorionic gonadotropin (hcg) treatment during the preovulatory period in gonadotropin-primed immature mice and rats. although cx3cr1, the seven-transmembrane receptor for fractalkine, was also found in murine ovaries, its transcripts displayed minimal changes. using tandem rt-pcr and immunohistochemistry, fractalkine transcripts and proteins were localized in cumulus, mural granulosa, and theca cells as well as the oocytes, whereas cx3cr1 was found in the same cells except the oocyte. real-time rt-pcr further indicated the hcg induction of fractalkine transcripts in different ovarian compartments, with the highest increases found in granulosa cells. in cultured granulosa cells, treatment with fractalkine augmented hcg stimulation of progesterone but not estradiol and camp biosynthesis with concomitant increases in transcript levels for key steroidogenic enzymes ( steroidogenic acute regulatory protein, cyp11a, and 3 beta-hydroxysteroid dehydrogenase). in cultured preovulatory follicles, treatment with fractalkine also augmented progesterone production stimulated by hcg. furthermore, treatment with fractalkine augmented the phosphorylation of p38 mapk in cultured granulosa cells. the present data demonstrated that increases in preovulatory lh/hcg induce the expression of fractalkine to augment the luteinization of preovulatory granulosa cells and suggest the fractalkine/cx3cr1 signaling system plays a potential paracrine/autocrine role in preovulatory follicles.
WOS关键词CYCLASE-ACTIVATING POLYPEPTIDE ; RAT OVARY ; PREOVULATORY FOLLICLES ; PARACRINE REGULATION ; GENE-EXPRESSION ; P38 MAPK ; INFLAMMATORY REACTION ; SIGNAL-TRANSDUCTION ; HORMONAL-REGULATION ; RECEPTOR CX(3)CR1
WOS研究方向Endocrinology & Metabolism
WOS类目Endocrinology & Metabolism
语种英语
WOS记录号WOS:000256053100015
出版者ENDOCRINE SOC
URI标识http://www.irgrid.ac.cn/handle/1471x/2387834
专题中国科学院大学
通讯作者Duan, En-Kui
作者单位1.Chinese Acad Sci, Inst Zool, Key Lab Reprod Biol, Beijing 100101, Peoples R China
2.Stanford Univ, Sch Med, Dept Obstet & Gynecol, Div Reprod Biol, Stanford, CA 94305 USA
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
4.Organon, NL-5349 AB Oss, Netherlands
推荐引用方式
GB/T 7714
Zhao, Ping,De, Ananya,Hu, Zeng,et al. Gonadotropin stimulation of ovarian fractalkine expression and fractalkine augmentation of progesterone biosynthesis by luteinizing granulosa cells[J]. Endocrinology,2008,149(6):2782-2789.
APA Zhao, Ping.,De, Ananya.,Hu, Zeng.,Li, Jing.,Mulders, Sabine M..,...&Hsueh, Aaron J. W..(2008).Gonadotropin stimulation of ovarian fractalkine expression and fractalkine augmentation of progesterone biosynthesis by luteinizing granulosa cells.Endocrinology,149(6),2782-2789.
MLA Zhao, Ping,et al."Gonadotropin stimulation of ovarian fractalkine expression and fractalkine augmentation of progesterone biosynthesis by luteinizing granulosa cells".Endocrinology 149.6(2008):2782-2789.

入库方式: iSwitch采集

来源:中国科学院大学

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。