Casein Kinase 2 interacts with human mitogen- and stress-activated protein kinase MSK1 and phosphorylates it at Multiple sites
文献类型：期刊论文
| 作者 | Shi, Yan1; Han, Guanghui2; Wu, Huiling1; Ye, Kan1; Tian, Zhipeng1; Wang, Jiaqi1; Shi, Huili1; Ye, Mingliang2; Zou, Hanfa2; Huo, Keke1 |
| 刊名 | bmb reports
 |
| 出版日期 | 2009-12-31 |
| 卷号 | 42期号:12页码:840-845 |
| 关键词 | CK2 Mass spectrometry MSK1 Phosphorylation Yeast two-hybrid |
| 产权排序 | 2;1 |
| 通讯作者 | 叶明亮 ; keke huo |
| 英文摘要 | mitogen- and stress-activated protein kinase (msk1) palys; a crucial role in the regulation of transcription downstream of extracellular-signal-regulated kinase1/2 (erk1/2) and mitogen-activated protein kinase p38. msk1 can be phosphorylated and activated in cells by both erk1/2 and p38 alpha. in this study, casein kinase 2 (ck2) was identified as a binding and regulatory partner for msk1. using the yeast two-hybrid system, msk1 was found to interact with the ck2 beta regulatory subunit of ck2. interactions between msk1 and the ck2 alpha catalytic subunit and ck2 beta subunit were demonstrated in vitro and in vivo. we further found that ck2 alpha can only interact with the c-terminal kinase domain of msk1. using site-directed mutagenesis assay and mass spectrometry, we identified five sites in the msk1 c-terminus that could be phosphorylated by ck2 in vitro: ser757, ser758, ser759, ser760 and thr793. of these, ser757, ser759, ser760 and thr793 were previously unknown. [bmb reports 2009; 42(12): 840-845] |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | creb ; ck2 ; subunits ; mediate ; search |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000273334900012 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/103083]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Fudan Univ, Inst Genet, State Key Lab Genet Engn, Sch Life Sci, Shanghai 200433, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Yan,Han, Guanghui,Wu, Huiling,et al. Casein Kinase 2 interacts with human mitogen- and stress-activated protein kinase MSK1 and phosphorylates it at Multiple sites[J]. bmb reports,2009,42(12):840-845. |
| APA | Shi, Yan.,Han, Guanghui.,Wu, Huiling.,Ye, Kan.,Tian, Zhipeng.,...&Huo, Keke.(2009).Casein Kinase 2 interacts with human mitogen- and stress-activated protein kinase MSK1 and phosphorylates it at Multiple sites.bmb reports,42(12),840-845. |
| MLA | Shi, Yan,et al."Casein Kinase 2 interacts with human mitogen- and stress-activated protein kinase MSK1 and phosphorylates it at Multiple sites".bmb reports 42.12(2009):840-845. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。