Separation of peptide diastereomers using CEC and a hydrophobic monolithic column
文献类型：期刊论文
| 作者 | Ludewig, Ronny1; Dong, Jing2; Zou, Hanfa2; Scriba, Gerhard K. E.1 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 33期号:8页码:1085-1089 |
| 关键词 | CEC Neutral hydrophobic monolith Peptide diastereomers Peptide separation |
| ISSN号 | 1615-9306 |
| 产权排序 | 2;2 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | a neutral hydrophobic monolith prepared by radical in situ copolymerization of lauryl methacrylate and ethylene dimethacylate has been evaluated for the cec separation of diastereomers of small peptides using acidic mobile phases containing acn as organic modifier. using an acidic mobile phase, the peptides migrated due to their own electrophoretic mobility. hydrophobic interactions with the stationary phase contributed to the separation. peptide mobility and resolution increased with increasing the acn content. retention times increased with the ph of the mobile phase. peak resolution increased with buffer ph and concentration. di- and tripeptides composed only of l-configured amino acids migrated faster than peptides containing d-amino acids. a mixture of isomeric asp tripeptides that could not be completely resolved by either cze or h plc as well as the 24mer peptides tetracosactide and (16)[d-lys]-tetracosactide could also be separated by cec on the hydrophobic monolith. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | capillary-electrophoresis ; stationary phases ; electrochromatography ; tripeptides ; stereoisomers ; degradation ; resolution ; constants ; products |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000277248400013 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/103119]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Univ Jena, Dept Pharmaceut & Med Chem, Sch Pharm, D-07743 Jena, Germany 2.Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ludewig, Ronny,Dong, Jing,Zou, Hanfa,et al. Separation of peptide diastereomers using CEC and a hydrophobic monolithic column[J]. journal of separation science,2010,33(8):1085-1089. |
| APA | Ludewig, Ronny,Dong, Jing,Zou, Hanfa,&Scriba, Gerhard K. E..(2010).Separation of peptide diastereomers using CEC and a hydrophobic monolithic column.journal of separation science,33(8),1085-1089. |
| MLA | Ludewig, Ronny,et al."Separation of peptide diastereomers using CEC and a hydrophobic monolithic column".journal of separation science 33.8(2010):1085-1089. |
入库方式： OAI收割
来源：大连化学物理研究所
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