Purification and structure analysis of antifreeze proteins from ammopiptanthus mongolicus
文献类型：期刊论文
| 作者 | Fei, Y. B.2; Cao, P. X.2,3; Gao, S. Q.2; Wang, B.1; Wei, L. B.2; Zhao, J.2; Chen, G.2; Wang, B. H.1 |
| 刊名 | Preparative biochemistry & biotechnology
 |
| 出版日期 | 2008 |
| 卷号 | 38期号:2页码:179-190 |
| 关键词 | Ammopiptanthus mongolicus Antifreeze proteins Circular dichroism spectroscopy Differential scanning calorimetry Inhibition of the growth of ice crystals Sequence analysis |
| ISSN号 | 1082-6068 |
| DOI | 10.1080/10826060701885126 |
| 通讯作者 | Wang, b. h.(caopengxiu@163.com) |
| 英文摘要 | We purified many kinds of antifreeze proteins with high activity from the leaves of ammopiptanthus mongolicus by several biochemical techniques. the antifreeze activities of these afps were measured by both osmometry and differential scanning calorimetry, and the inhibition of growth of ice crystals by the afps was obvious. additionally, the antifreeze proteins were analyzed by sequencing, glycosylation reaction, mass spectroscopy, and circular dichroism spectroscopy. both samples have some other unique structures different from those of fishes and of insects. it was suggested that plant afps might have a particular antifreeze mechanism in comparison with that of fish and insects. |
| WOS关键词 | TOLERANCE ; STRESS ; GENE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000253732600006 |
| 出版者 | TAYLOR & FRANCIS INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2388091 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, B. H. |
| 作者单位 | 1.Peking Univ, Inst Phys Chem, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fei, Y. B.,Cao, P. X.,Gao, S. Q.,et al. Purification and structure analysis of antifreeze proteins from ammopiptanthus mongolicus[J]. Preparative biochemistry & biotechnology,2008,38(2):179-190. |
| APA | Fei, Y. B..,Cao, P. X..,Gao, S. Q..,Wang, B..,Wei, L. B..,...&Wang, B. H..(2008).Purification and structure analysis of antifreeze proteins from ammopiptanthus mongolicus.Preparative biochemistry & biotechnology,38(2),179-190. |
| MLA | Fei, Y. B.,et al."Purification and structure analysis of antifreeze proteins from ammopiptanthus mongolicus".Preparative biochemistry & biotechnology 38.2(2008):179-190. |
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来源：中国科学院大学
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