Molecular identification and functional characterization of a drosophila dual-specificity phosphatase dmkp-4 which is involved in pgn-induced activation of the jnk pathway
文献类型：期刊论文
| 作者 | Sun, Lei1,2,3; Yu, Ming-Can1,2; Kong, Ling1,2,3; Zhuang, Zi-Heng1,2,3; Hu, Jun-Hao1,2,3; Ge, Bao-Xue1,2 |
| 刊名 | Cellular signalling
 |
| 出版日期 | 2008-07-01 |
| 卷号 | 20期号:7页码:1329-1337 |
| 关键词 | Mapk phosphatases (mkps) Dmkp-4 Map (mitogen-activated protein) kinases Djnk |
| ISSN号 | 0898-6568 |
| 通讯作者 | Ge, bao-xue(gebaoxue@sibs.ac.cn) |
| 英文摘要 | Map (mitogen-activated protein) kinases play an important role in regulating many critical cellular processes. the inactivation of map kinases is always accomplished by a family of dual-specificity phosphatases, termed mapk phosphatases (mkps). here, we have identified a novel mkp-like protein, designated dmkp-4, from the drosophila genome. dmkp-4 is a protein of 387 amino acids, with a dual-specificity phosphatase (dsp) catalytic domain. recombinant protein dmkp-4 retains intrinsic phosphatase activity against chromogenic substrate pnpp. overexpression of dmkp-4 inhibited the activation of erk, jnk and p38 by h2o2, sorbitol and heat shock in hek293-t cells, and jnk activation in drosophila s2* cells under pgn stimuli. "knockdown" of dmkp-4 expression by rnai significantly enhanced the pcn-stimulated activation of jnk, but not erk nor p38. further study revealed that dmkp-4 interacted specifically with jnk via its dsp domain. mutation of cys-126 to serine in the dsp domain of dmkp-4 not only eliminated its interaction with jnk, but also markedly reduced its phosphatase activity. thus, dmkp-4 is a drosophila homologue of mammalian mkps, and may play important roles in the regulation of various developmental processes. (c) 2008 elsevier inc. all rights reserved. |
| WOS关键词 | PROTEIN-KINASE PHOSPHATASE-3 ; SIGNAL-REGULATED KINASE ; MAPK PHOSPHATASE ; IMMUNE-RESPONSE ; PEPTIDOGLYCAN RECOGNITION ; CATALYTIC ACTIVATION ; IMD-PATHWAY ; P38 ; BINDING ; GENE |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000257013000012 |
| 出版者 | ELSEVIER SCIENCE INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2388096 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ge, Bao-Xue |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Lab Signal Transduct, Shanghai 200025, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Shanghai 200025, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Lei,Yu, Ming-Can,Kong, Ling,et al. Molecular identification and functional characterization of a drosophila dual-specificity phosphatase dmkp-4 which is involved in pgn-induced activation of the jnk pathway[J]. Cellular signalling,2008,20(7):1329-1337. |
| APA | Sun, Lei,Yu, Ming-Can,Kong, Ling,Zhuang, Zi-Heng,Hu, Jun-Hao,&Ge, Bao-Xue.(2008).Molecular identification and functional characterization of a drosophila dual-specificity phosphatase dmkp-4 which is involved in pgn-induced activation of the jnk pathway.Cellular signalling,20(7),1329-1337. |
| MLA | Sun, Lei,et al."Molecular identification and functional characterization of a drosophila dual-specificity phosphatase dmkp-4 which is involved in pgn-induced activation of the jnk pathway".Cellular signalling 20.7(2008):1329-1337. |
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来源：中国科学院大学
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