Shotgun approach based comparative proteomic analysis of levo-tetrahydropalmatine-induced apoptosis in hepatocytes
文献类型：期刊论文
| 作者 | Wang, Chen1,2,4; Zhou, Jiangrui3; Wang, Shuowen1,4; Ye, Mingliang2; Fan, Guorong1,4; Zou, Hanfa2; Jiang, Chunlei3 |
| 刊名 | toxicology letters
 |
| 出版日期 | 2010-04-15 |
| 卷号 | 194期号:1-2页码:8-15 |
| 关键词 | 2D-nano-LC Proteomics Tetrahydropalmatine Toxicity |
| ISSN号 | 0378-4274 |
| 产权排序 | 2;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | the analgesic agent levo-tetrahydropalmatine (l-thp) was reported to be associated with acute or chronic hepatitis in clinical practice. we found that l-thp can induce apoptosis in the hepatocytes of balb/c mice and human normal liver l-02 (l-02) cells. several key molecules, including caspase-3, bcl-2. bad and bax, were modulated by l-thp treatment. a novel high-throughput proteomic approach based on 2d-nano-lc-ms/ms was applied to simultaneously evaluate the alterations of global protein expression involved in the response of l-thp treatment in l-02 cells. a total of 156 deregulated proteins were identified, among which 12 proteins play regulatory or constitutive roles in the apoptosis pathways. further analyses of two proteins (mtor and mek2) by western blots confirmed that these proteins were expressed at lower levels in l-thp-treated l-02 cells compared with those of control. the current study provided detailed evidence to support that l-thp is capable of inducing apoptosis in mammalian liver cells and improve the understanding of mechanisms of l-thp-induced hepatotoxicity. crown copyright (c) 2010 published by elsevier ireland ltd. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | toxicology |
| 研究领域[WOS] | toxicology |
| 关键词[WOS] | induced liver-injury ; jin bu huan ; drug-induced hepatotoxicity ; l-02 cells ; cancer cells ; protein ; mechanisms ; expression ; growth ; mtor |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000276933400002 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/103121]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Second Mil Med Univ, Sch Pharm, Dept Pharmaceut Anal, Shanghai 200433, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 3.Second Mil Med Univ, Dept Naut Med, Lab Stress Med, Shanghai 200433, Peoples R China 4.Shanghai Key Lab Pharmaceut Metabolite Res, Shanghai 200433, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chen,Zhou, Jiangrui,Wang, Shuowen,et al. Shotgun approach based comparative proteomic analysis of levo-tetrahydropalmatine-induced apoptosis in hepatocytes[J]. toxicology letters,2010,194(1-2):8-15. |
| APA | Wang, Chen.,Zhou, Jiangrui.,Wang, Shuowen.,Ye, Mingliang.,Fan, Guorong.,...&Jiang, Chunlei.(2010).Shotgun approach based comparative proteomic analysis of levo-tetrahydropalmatine-induced apoptosis in hepatocytes.toxicology letters,194(1-2),8-15. |
| MLA | Wang, Chen,et al."Shotgun approach based comparative proteomic analysis of levo-tetrahydropalmatine-induced apoptosis in hepatocytes".toxicology letters 194.1-2(2010):8-15. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。