Combined Comparative and Chemical Proteomics on the Mechanisms of levo-Tetrahydropalmatine-Induced Antinociception in the Formalin Test
文献类型：期刊论文
| 作者 | Wang, Chen1,2,4; Zhou, Jiangrui3; Wang, Shuowen1,4; Ye, Mingliang2; Jiang, Chunlei3; Fan, Guorong1,4; Zou, Hanfa2 |
| 刊名 | journal of proteome research
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 9期号:6页码:3225-3234 |
| 关键词 | 2-D nano LC proteomics chemoproteomics tetrahydropalmatine |
| ISSN号 | 1535-3893 |
| 产权排序 | 2;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | this study investigated the mechanisms involved in the antinociceptive action induced by levo-tetrahydropalmatine (l-thp) in the formalin test by combined comparative and chemical proteomics. rats were pretreated with l-thp by the oral route (40 mg/kg) 1 h before formalin injection. the antinociceptive effect of l-thp was shown in the first and second phases of the formalin test. to address the mechanisms by which l-thp inhibits formalin-induced nociception in rats, the combined comparative and chemical proteomics were applied. a novel high-throughput comparative proteomic approach based on 2d-nano-lc-ms/ms was applied to simultaneously evaluate the deregulated proteins involved in the response of l-thp treatment in formalin-induced pain rats. thousands of proteins were identified, among which 17 proteins survived the stringent filter criteria and were further included for functional discussion. two proteins (neurabin-1 and calcium-dependent secretion activator 1) were randomly selected, and their expression levels were further confirmed by western blots. the results matched well with those of proteomics. in the present study, we also described the development and application of l-thp immobilized beads to bind the targets. following incubation with cellular lysates, the proteome interacting with the fixed l-thp was identified. the results of comparative and chemical proteomics were quite complementary. although the precise roles of these identified moleculars in l-thp-induced antinociception need further study, the combined results indicated that proteins associated with signal transduction, vesicular trafficking and neurotransmitter release, energy metabolism, and ion transport play important roles in l-thp-induced antinociception in the formalin test. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | actin-depolymerizing protein ; dense-core vesicles ; neuropathic pain ; phosphoinositide 3-kinase ; biomarker discovery ; dopamine-receptors ; activator protein ; fos expression ; proton pumps ; human-brain |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000278243300041 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/103125]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Second Mil Med Univ, Sch Pharm, Dept Pharmaceut Anal, Shanghai 200433, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 3.Second Mil Med Univ, Dept Naut Med, Lab Stress Med, Shanghai 200433, Peoples R China 4.Shanghai Key Lab Pharmaceut Metabolite, Shanghai 200433, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chen,Zhou, Jiangrui,Wang, Shuowen,et al. Combined Comparative and Chemical Proteomics on the Mechanisms of levo-Tetrahydropalmatine-Induced Antinociception in the Formalin Test[J]. journal of proteome research,2010,9(6):3225-3234. |
| APA | Wang, Chen.,Zhou, Jiangrui.,Wang, Shuowen.,Ye, Mingliang.,Jiang, Chunlei.,...&Zou, Hanfa.(2010).Combined Comparative and Chemical Proteomics on the Mechanisms of levo-Tetrahydropalmatine-Induced Antinociception in the Formalin Test.journal of proteome research,9(6),3225-3234. |
| MLA | Wang, Chen,et al."Combined Comparative and Chemical Proteomics on the Mechanisms of levo-Tetrahydropalmatine-Induced Antinociception in the Formalin Test".journal of proteome research 9.6(2010):3225-3234. |
入库方式： OAI收割
来源：大连化学物理研究所
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