Isolation of six novel aquaporin genes from triticum aestivum l. and functional analysis of taaqp6 in water redistribution
文献类型：期刊论文
| 作者 | Zhang, Junfeng1,2; Deng, Zhiyong1; Cao, Shuanghe1; Wang, Xianping1; Zhang, Aimin1; Zhang, Xiangqi1 |
| 刊名 | Plant molecular biology reporter
 |
| 出版日期 | 2008-03-01 |
| 卷号 | 26期号:1页码:32-45 |
| 关键词 | Aquaporin Gene expression Gene isolation Osmotic stress Water redistribution Wheat |
| ISSN号 | 0735-9640 |
| DOI | 10.1007/s11105-008-0020-0 |
| 通讯作者 | Zhang, xiangqi(xqzhang@genetics.ac.cn) |
| 英文摘要 | Water transport through the cell membranes is regulated largely by a family of proteins known as aquaporins (aqps). in this study, six novel aquaporin genes, taaqp1 similar to taaqp6, were isolated from the common wheat triticum aestivum l. var. changle 5. these six taaqps share common features with other plant aqps. three of them (taaqp2, 3, and 4) phylogenetically belong to plant pip2 group, and the other three resemble proteins from pip1(taaqp1), tip1(taaqp6), and tip2 (taaqp5) groups, respectively. the subcellular localization assay, determined by onion epidermal cell transient expression system, indicated that taaqp6 is a membrane-associated protein. transformation of taaqp6 into xenopus oocytes demonstrated that it facilitated water uptake, suggesting that this taaqp is an active membrane-associated protein. the expression of taaqp6 was upregulated in shoots of wheat seedlings, but downregulated in roots under polyethylene glycol (peg) stress. in split-root experiment, the amount of taaqp6 transcripts decreased in dry roots (cultured in peg solution), but increased in wet roots (cultured in water), and remained unchanged in shoots. it is interesting to note that the osmolality changes of different parts in peg-treated and split-root seedlings were related to the expression of taaqp6. taken together, we proposed that taaqp6 participates in internal water redistribution of wheat seedlings during osmotic stress by regulating outflow of water from water-rich organs to the parts with poor water status under water deficit condition. |
| WOS关键词 | MAJOR INTRINSIC PROTEINS ; ARABIDOPSIS-THALIANA ; MEMBRANE AQUAPORIN ; CHANNEL PROTEINS ; PLANT AQUAPORINS ; EXPRESSION ; FAMILY ; ROOTS ; TRANSPORT ; DROUGHT |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemical Research Methods ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000259962400003 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2388241 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, Xiangqi |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Junfeng,Deng, Zhiyong,Cao, Shuanghe,et al. Isolation of six novel aquaporin genes from triticum aestivum l. and functional analysis of taaqp6 in water redistribution[J]. Plant molecular biology reporter,2008,26(1):32-45. |
| APA | Zhang, Junfeng,Deng, Zhiyong,Cao, Shuanghe,Wang, Xianping,Zhang, Aimin,&Zhang, Xiangqi.(2008).Isolation of six novel aquaporin genes from triticum aestivum l. and functional analysis of taaqp6 in water redistribution.Plant molecular biology reporter,26(1),32-45. |
| MLA | Zhang, Junfeng,et al."Isolation of six novel aquaporin genes from triticum aestivum l. and functional analysis of taaqp6 in water redistribution".Plant molecular biology reporter 26.1(2008):32-45. |
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来源：中国科学院大学
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