A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum
文献类型：期刊论文
| 作者 | Zhu Jun; Wang FangJun; Dong XiaoLi; Ye MingLiang; Zou HanFa |
| 刊名 | science china-chemistry
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 53期号:4页码:759-767 |
| 关键词 | peptidomics label-free quantification hepatocellular carcinoma breast cancer human serum |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | peptidomics draws more and more attention in discovering useful biomarkers for early diagnosis of disease. however, there is lack of efficient quantification strategy in peptidome analysis. in this study, a strategy with label-free quantification of the targeted endogenous peptides based on peak intensity using mu uplc-q-tof-ms/ms was developed for quantitative peptidome analysis of human serum. different amounts of standard bsa tryptic digesting peptides were added into the same serum extracts for evaluation of the developed strategy, and it was observed that the average relative error of the targeted peptides was 6.42%, which was superior to the result obtained directly by commercially available software plgs. it was also demonstrated that this quantification strategy could obviously increase the detection sensitivity of the peptide by dda analysis. then, this strategy was applied to comparatively analyze the peptides extracted from the serum of hcc or breast cancer patients and healthy individuals, respectively. peptides with charge states up to 5 and molecular weight over 4000 can be reliably identified and quantified. this quantitative analysis method based on. mu uplc-q-tof-ms/ms exhibited superior sensitivity than that by maldi-tof-ms commonly used in peptidome analysis. finally, some interesting endogenous peptides related to corresponding diseases were successfully obtained. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | mesoporous silica particles ; tandem mass-spectrometry ; biomarker discovery ; proteomic patterns ; cancer ; protein ; disease ; identification ; metabolism ; enrichment |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000278263400009 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/103153]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu Jun,Wang FangJun,Dong XiaoLi,et al. A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum[J]. science china-chemistry,2010,53(4):759-767. |
| APA | Zhu Jun,Wang FangJun,Dong XiaoLi,Ye MingLiang,&Zou HanFa.(2010).A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum.science china-chemistry,53(4),759-767. |
| MLA | Zhu Jun,et al."A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum".science china-chemistry 53.4(2010):759-767. |
入库方式： OAI收割
来源：大连化学物理研究所
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