Genome shuffling in the ethanologenic yeast candida krusei to improve acetic acid tolerance
文献类型：期刊论文
| 作者 | Wei, Pingying1,2; Li, Zilong1; He, Peng1; Lin, Yuping1; Jiang, Ning1 |
| 刊名 | Biotechnology and applied biochemistry
 |
| 出版日期 | 2008-02-01 |
| 卷号 | 49页码:113-120 |
| ISSN号 | 0885-4513 |
| DOI | 10.1042/ba20070072 |
| 通讯作者 | Jiang, ning(jiangn@im.ac.cn) |
| 英文摘要 | Genome shuffling was used to improve the acetic acid tolerance of an ethanologenic yeast, candida krusei gl560. a mutant, s4-3, was isolated and selected after four rounds of genome shuffling. it was found that the mutant s4-3 had a higher viability in the ynbx (yeast nitrogen base/xylose) medium with acetic acid and grew better in the ypd (yeast extract, peptone and dextrose) medium [1 % (w/v) yeast extract, 2% (w/v) peptone and 2 % (w/v) glucose] with acetic acid than the parent strain gls60. the mutant s4-3 also improved its multiple stress tolerance to ethanol, h2o2, heat and freeze-thaw. furthermore, s4-3 showed higher ethanol production than gl560 in efm (ethanol fermentation medium) with or without acetic acid. the dna content of s4-3 was similar to its parent strains in the genome shuffling. this suggested that gene exchange, as caused by homologous recombination, may have occurred during the process. higher membrane integrity and intracellular catalase activity were two possible reasons for the higher acid-tolerance phenotype of s4-3. these results indicated that genome shuffling is a powerful means of rapidly improving the complex traits of non-haploid organisms, while still maintaining robust growth. |
| WOS关键词 | SACCHAROMYCES-CEREVISIAE ; LACTIC-ACID ; PHENOTYPIC IMPROVEMENT ; PROTOPLAST FUSION ; TREHALOSE CONTENT ; ABC TRANSPORTER ; PICHIA-STIPITIS ; ORGANIC-ACIDS ; CORN MASH ; ADAPTATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000253080900003 |
| 出版者 | PORTLAND PRESS LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2388364 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jiang, Ning |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Pingying,Li, Zilong,He, Peng,et al. Genome shuffling in the ethanologenic yeast candida krusei to improve acetic acid tolerance[J]. Biotechnology and applied biochemistry,2008,49:113-120. |
| APA | Wei, Pingying,Li, Zilong,He, Peng,Lin, Yuping,&Jiang, Ning.(2008).Genome shuffling in the ethanologenic yeast candida krusei to improve acetic acid tolerance.Biotechnology and applied biochemistry,49,113-120. |
| MLA | Wei, Pingying,et al."Genome shuffling in the ethanologenic yeast candida krusei to improve acetic acid tolerance".Biotechnology and applied biochemistry 49(2008):113-120. |
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来源：中国科学院大学
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