Cloning and characterization of a heat shock protein 70 gene, mshsp70-1, in medicago sativa
文献类型：期刊论文
| 作者 | He, Zhishui1,2; Xie, Rong1,2; Wang, Yanzhang1; Zou, Huasong1; Zhu, Jiabi1; Yu, Guanqiao1 |
| 刊名 | Acta biochimica et biophysica sinica
 |
| 出版日期 | 2008-03-01 |
| 卷号 | 40期号:3页码:209-216 |
| 关键词 | Heat shock protein 70 Cdna array Symbiotic nitrogen fixation Nodule development |
| ISSN号 | 1672-9145 |
| DOI | 10.1111/j.1745-7270.2008.00394.x |
| 通讯作者 | Yu, guanqiao(gqyu@sippe.ac.cn) |
| 英文摘要 | Some members of the heat shock protein 70 (hsp70) family have important functions in organism development. here, we identified an alfalfa (medicago sativa l. cv. algonquin) hsp70 gene, mshsp70-1, using cdna array and reverse transcription-polymerase chain reaction. this gene contains a 1947 bp open reading frame encoding a protein of 649 amino acids. this protein contains all conserved domains, motifs, and characteristic sequences of plant hsp70s. the expression of mshsp70-1 is enhanced in nodule compared with root, stem, leaf, and flower, and throughout the process of nodule development. northern hybridization analysis indicated that the expression of mshsp70-1 in nodule requires the active baca gene of rhizobia. these results suggested that mshsp70-1 might play an important role in alfalfanodule development. |
| WOS关键词 | HEAT-SHOCK PROTEINS ; MOLECULAR CHAPERONES ; NODULE DEVELOPMENT ; EXPRESSION ; MELILOTI ; ARABIDOPSIS ; TRUNCATULA ; FAMILY ; HSP70 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000254188800004 |
| 出版者 | BLACKWELL PUBLISHING |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2388488 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yu, Guanqiao |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Lab Plant Mol Genet, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Zhishui,Xie, Rong,Wang, Yanzhang,et al. Cloning and characterization of a heat shock protein 70 gene, mshsp70-1, in medicago sativa[J]. Acta biochimica et biophysica sinica,2008,40(3):209-216. |
| APA | He, Zhishui,Xie, Rong,Wang, Yanzhang,Zou, Huasong,Zhu, Jiabi,&Yu, Guanqiao.(2008).Cloning and characterization of a heat shock protein 70 gene, mshsp70-1, in medicago sativa.Acta biochimica et biophysica sinica,40(3),209-216. |
| MLA | He, Zhishui,et al."Cloning and characterization of a heat shock protein 70 gene, mshsp70-1, in medicago sativa".Acta biochimica et biophysica sinica 40.3(2008):209-216. |
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来源：中国科学院大学
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