A facile and efficient strategy for one-step in situ preparation of hydrophobic organic monolithic stationary phases by click chemistry and its application on protein separation
文献类型：期刊论文
| 作者 | Sun, Xiangli1; Lin, Dong1; He, Xiwen1; Chen, Langxing1; Zhang, Yukui1,2 |
| 刊名 | talanta
 |
| 出版日期 | 2010-06-30 |
| 卷号 | 82期号:1页码:404-408 |
| 关键词 | Click chemistry High performance liquid chromatography In situ Polymer monolithic column Protein separation |
| 产权排序 | 2;5 |
| 通讯作者 | 陈朗星 ; 张玉奎 |
| 英文摘要 | a simple one-step in situ "click" modification strategy was developed for the preparation of hydrophobic organic monolithic columns for the first time. the column morphology and surface chemistry of the fabricated monolithic columns were characterized by scanning electron microscopy, fourier transform infrared spectroscopy and x-ray photoelectron spectroscopy, respectively. the chromatographic performances of the c8/c18 "click" monoliths were evaluated through the separation of a mixture of five proteins such as ribonuclease a, soybean trypsin inhibitor, cytochrome c, bovine haemoglobin and bovine serum albumin. compared with the blank column, the higher hydrophobicity stationary phases obtained from the "clicked" modification have longer retention times and higher resolution for the five proteins. the separation of five proteins mixture on click c18 monolith with gradient elution at different flow rates was also investigated, the baseline separation of five proteins could be achieved at three different flow rates. (c) 2010 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; alkyne 3+2 cycloaddition ; continuous polymer beds ; silica ; columns ; azides ; functionalization ; stabilization ; peptides ; ligation |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000279488900058 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/103211]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Xiangli,Lin, Dong,He, Xiwen,et al. A facile and efficient strategy for one-step in situ preparation of hydrophobic organic monolithic stationary phases by click chemistry and its application on protein separation[J]. talanta,2010,82(1):404-408. |
| APA | Sun, Xiangli,Lin, Dong,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2010).A facile and efficient strategy for one-step in situ preparation of hydrophobic organic monolithic stationary phases by click chemistry and its application on protein separation.talanta,82(1),404-408. |
| MLA | Sun, Xiangli,et al."A facile and efficient strategy for one-step in situ preparation of hydrophobic organic monolithic stationary phases by click chemistry and its application on protein separation".talanta 82.1(2010):404-408. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。