The silkworm (bombyx mori) micrornas and their expressions in multiple developmental stages
文献类型：期刊论文
| 作者 | Yu, Xiaomin1,2; Zhou, Qing1,2; Li, Sung-Chou4,5; Luo, Qibin1,3; Cai, Yimei1,2; Lin, Wen-chang4,6; Chen, Huan1,3; Yang, Yue1,2; Hu, Songnian1; Yu, Jun1 |
| 刊名 | Plos one
 |
| 出版日期 | 2008-08-20 |
| 卷号 | 3期号:8页码:11 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0002997 |
| 通讯作者 | Yu, xiaomin() |
| 英文摘要 | Background: micrornas (mirnas) play crucial roles in various physiological processes through post-transcriptional regulation of gene expressions and are involved in development, metabolism, and many other important molecular mechanisms and cellular processes. the bombyx mori genome sequence provides opportunities for a thorough survey for mirnas as well as comparative analyses with other sequenced insect species. methodology/principal findings: we identified 114 non-redundant conserved mirnas and 148 novel putative mirnas from the b. mori genome with an elaborate computational protocol. we also sequenced 6,720 clones from 14 developmental stage-specific small rna libraries in which we identified 35 unique mirnas containing 21 conserved mirnas (including 17 predicted mirnas) and 14 novel mirnas (including 11 predicted novel mirnas). among the 114 conserved mirnas, we found six pairs of clusters evolutionarily conserved cross insect lineages. our observations on length heterogeneity at 59 and/or 39 ends of nine mirnas between cloned and predicted sequences, and three mature forms deriving from the same arm of putative pre-mirnas suggest a mechanism by which mirnas gain new functions. analyzing development-related mirnas expression at 14 developmental stages based on clone-sampling and stem-loop rt pcr, we discovered an unusual abundance of 33 sequences representing 12 different mirnas and sharply fluctuated expression of mirnas at larva-molting stage. the potential functions of several stage-biased mirnas were also analyzed in combination with predicted target genes and silkworm's phenotypic traits; our results indicated that mirnas may play key regulatory roles in specific developmental stages in the silkworm, such as ecdysis. conclusions/significance: taking a combined approach, we identified 118 conserved mirnas and 151 novel mirna candidates from the b. mori genome sequence. our expression analyses by sampling mirnas and real-time pcr over multiple developmental stages allowed us to pinpoint molting stages as hotspots of mirna expression both in sorts and quantities. based on the analysis of target genes, we hypothesized that mirnas regulate development through a particular emphasis on complex stages rather than general regulatory mechanisms. |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000264420900022 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2388698 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yu, Xiaomin |
| 作者单位 | 1.Chinese Acad Sci, Beijing Inst Genom, Key Lab Genome Sci & Informat, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100864, Peoples R China 3.Zhejiang Univ, Watson Inst Genome Sci, Hangzhou, Peoples R China 4.Natl Yang Ming Univ, Inst BioMed Informat, Taipei, Taiwan 5.Acad Sinica, Taiwan Int Grad Program, Bioinformat Program, Taipei, Taiwan 6.Acad Sinica, Inst Biomed Sci, Taipei, Taiwan |
| 推荐引用方式 GB/T 7714 | Yu, Xiaomin,Zhou, Qing,Li, Sung-Chou,et al. The silkworm (bombyx mori) micrornas and their expressions in multiple developmental stages[J]. Plos one,2008,3(8):11. |
| APA | Yu, Xiaomin.,Zhou, Qing.,Li, Sung-Chou.,Luo, Qibin.,Cai, Yimei.,...&Yu, Jun.(2008).The silkworm (bombyx mori) micrornas and their expressions in multiple developmental stages.Plos one,3(8),11. |
| MLA | Yu, Xiaomin,et al."The silkworm (bombyx mori) micrornas and their expressions in multiple developmental stages".Plos one 3.8(2008):11. |
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来源：中国科学院大学
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