Cloning and expression analysis of rice sucrose transporter genes ossut2m and ossut5z
文献类型：期刊论文
| 作者 | Sun, Ai-Jun1,2; Xu, Hong-Lin1; Gong, Wan-Kui1,2; Zhai, Hong-Li1,2; Meng, Kun1; Wang, Yong-Qin1,2; Wei, Xiao-Li1; Xiao, Gui-Fang1; Zhu, Zhen1 |
| 刊名 | Journal of integrative plant biology
 |
| 出版日期 | 2008 |
| 卷号 | 50期号:1页码:62-75 |
| 关键词 | Cdna cloning Expression pattern Ossut2m Ossut5z Rice sucrose transporter |
| ISSN号 | 1672-9072 |
| DOI | 10.1111/j.1744-7909.2007.00596.x |
| 通讯作者 | Zhu, zhen(zhuzhen@cashq.ac.cn) |
| 英文摘要 | Two sucrose transporter (sut) cdnas, ossut2m and ossut5z, were isolated from rice ( oryza sativa l.) by reverse transcription polymerase chain reaction (rt-pcr). sequencing results indicate they are 1 531 bp and 1 635 bp in length including complete open reading frame 1 506 bp and 1 608 bp, which encode 502 amino acids and 536 amino acids, respectively. the toppred program suggested that both sucrose transporter proteins, ossut2m and ossut5z, consist of potentially 12 transmembrane domains. semi-quantitative rt-pcr was carried out to investigate the gene expression patterns of ossut2m and ossut5z. in vegetative organs, transcripts of ossut2m were higher in source leaf blades than in other organs at the same development stage, whereas transcripts of ossut5z were less traceable in all organs investigated. in reproductive organs, both transcripts of these two genes were high in panicles from the booting stage to 7 days after flowering (daf) and then sharply declined. the potential physiology functions of these two sucrose transporters are discussed. |
| WOS关键词 | ENUCLEATE SIEVE ELEMENTS ; PLANTAGO-MAJOR L ; ORYZA-SATIVA L. ; SEED DEVELOPMENT ; MOLECULAR CHARACTERIZATION ; SUGAR TRANSPORTERS ; PHLOEM ; CARRIER ; IDENTIFICATION ; ARABIDOPSIS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000252209300008 |
| 出版者 | WILEY-BLACKWELL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2388863 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhu, Zhen |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Ai-Jun,Xu, Hong-Lin,Gong, Wan-Kui,et al. Cloning and expression analysis of rice sucrose transporter genes ossut2m and ossut5z[J]. Journal of integrative plant biology,2008,50(1):62-75. |
| APA | Sun, Ai-Jun.,Xu, Hong-Lin.,Gong, Wan-Kui.,Zhai, Hong-Li.,Meng, Kun.,...&Zhu, Zhen.(2008).Cloning and expression analysis of rice sucrose transporter genes ossut2m and ossut5z.Journal of integrative plant biology,50(1),62-75. |
| MLA | Sun, Ai-Jun,et al."Cloning and expression analysis of rice sucrose transporter genes ossut2m and ossut5z".Journal of integrative plant biology 50.1(2008):62-75. |
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来源：中国科学院大学
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