Optimization of mature embryo-based high frequency callus induction and plant regeneration from elite wheat cultivars grown in china
文献类型：期刊论文
| 作者 | Yu, Y.1,2; Wang, J.1,2; Zhu, M. -L.1,2; Wei, Z. -M.1 |
| 刊名 | Plant breeding
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 127期号:3页码:249-255 |
| 关键词 | Triticum aestivum Embryogenic callus In vitro regeneration Silver nitrate Cupric sulfate |
| ISSN号 | 0179-9541 |
| DOI | 10.1111/j.1439-0523.2007.01461.x |
| 通讯作者 | Wei, z. -m.(zmwei@sibs.ac.cn) |
| 英文摘要 | We have developed an efficient procedure for plant regeneration of elite wheat cultivars using mature embryos. firstly, we established the optimal combination of basal media, inoculation method and pretreatment method using biostatistical methods. the results indicated that the combination of ms medium and longitudinally bisected mature embryos showed the highest culture efficiency, whereas the pretreatment method had no significant effects on callus induction or plant regeneration. a 70% primary callus induction rate was achieved on ms medium containing 2 mg/l 2,4-d for all tested cultivars. primary calli were then transferred onto the subculture medium to initiate embryogenic calli. supplementation of the subculture medium with the appropriate combination of phytohormones (2.0 mg/l 2,4-d, 0.5 mg/l ba and 0.1 mg/l naa) significantly enhanced embryogenic callus production. the addition of agno(3) (10 mg/l) in regeneration medium promoted plant regeneration, whereas cuso(4) stimulated root formation. the use of this protocol achieved successful plant regeneration in eight tested cultivars. the culture efficiency ranged from 15.3% to 36.8%, suggesting this regeneration system may be an effective alternative for wheat genetic transformation. |
| WOS关键词 | TRITICUM-AESTIVUM L ; TOBACCO TISSUE-CULTURES ; BARLEY HORDEUM-VULGARE ; SOMATIC EMBRYOGENESIS ; ANTHER CULTURE ; ETHYLENE INHIBITOR ; SHOOT REGENERATION ; WINTER-WHEAT ; IN-VITRO ; INITIATION |
| WOS研究方向 | Agriculture ; Biotechnology & Applied Microbiology ; Plant Sciences |
| WOS类目 | Agronomy ; Biotechnology & Applied Microbiology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000255287500006 |
| 出版者 | WILEY-BLACKWELL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2388959 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wei, Z. -M. |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Y.,Wang, J.,Zhu, M. -L.,et al. Optimization of mature embryo-based high frequency callus induction and plant regeneration from elite wheat cultivars grown in china[J]. Plant breeding,2008,127(3):249-255. |
| APA | Yu, Y.,Wang, J.,Zhu, M. -L.,&Wei, Z. -M..(2008).Optimization of mature embryo-based high frequency callus induction and plant regeneration from elite wheat cultivars grown in china.Plant breeding,127(3),249-255. |
| MLA | Yu, Y.,et al."Optimization of mature embryo-based high frequency callus induction and plant regeneration from elite wheat cultivars grown in china".Plant breeding 127.3(2008):249-255. |
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来源：中国科学院大学
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