Identification of protein ubiquitination by ip-2d nano-hplc-maldi-tof-tof
文献类型：期刊论文
| 作者 | Mao Jian-Hua1,2,3,4; Zhang Qun-Ye2,3; Huang Qiu-Hua2,3; Chen Zhu2,3; Chen Quan1; Chen Sai-Juan1,2,3 |
| 刊名 | Progress in biochemistry and biophysics
 |
| 出版日期 | 2008-07-01 |
| 卷号 | 35期号:7页码:839-847 |
| 关键词 | Ubiquitination Ptm Ip-2d nano-hplc-maldi-tof-tof |
| ISSN号 | 1000-3282 |
| 通讯作者 | Chen sai-juan(sjchen@stn.sh.cn) |
| 英文摘要 | Mass spectrometry has been extensively used in the identification of protein and its posttranslational modification (ptm). mass spectrometry coupled with nano high performance liquid chromatography (hplc) could improve its resolution and efficiency. ubiquitination, one kind of the important protein posttranslational modifications (ptm), plays a pivotal role in dynamic balance and functional regulation of protein. a new strategy taking the advantage of immunoprecipitation, 2d nano hplc and matrix-assisted laser desorption/ionization-time of flight mass spectrometry was established to identify the ubiquitination of endogenous protein in mammalian cells. by using this strategy, the ubiquitinated sites of c-abl in k562 leukemia cells was successfully identified. thus, it may provide a valuable tool to characterizing the ubiquitination of endogenous proteins under physiologic and pathologic conditions. |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; POSTTRANSLATIONAL MODIFICATIONS ; PROTEOMIC IDENTIFICATION ; PEPTIDES ; SYSTEM ; MECHANISMS ; CELLS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000257888000017 |
| 出版者 | CHINESE ACAD SCIENCES, INST BIOPHYSICS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2389015 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen Sai-Juan |
| 作者单位 | 1.Chinese Acad Sci, Inst Zool, Beijing 100101, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, State Key Lab Med Genom, Shanghai 200025, Peoples R China 3.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Shanghai Inst Hematol, Shanghai 200025, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mao Jian-Hua,Zhang Qun-Ye,Huang Qiu-Hua,et al. Identification of protein ubiquitination by ip-2d nano-hplc-maldi-tof-tof[J]. Progress in biochemistry and biophysics,2008,35(7):839-847. |
| APA | Mao Jian-Hua,Zhang Qun-Ye,Huang Qiu-Hua,Chen Zhu,Chen Quan,&Chen Sai-Juan.(2008).Identification of protein ubiquitination by ip-2d nano-hplc-maldi-tof-tof.Progress in biochemistry and biophysics,35(7),839-847. |
| MLA | Mao Jian-Hua,et al."Identification of protein ubiquitination by ip-2d nano-hplc-maldi-tof-tof".Progress in biochemistry and biophysics 35.7(2008):839-847. |
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来源：中国科学院大学
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