Selective extraction of low molecular weight proteins by mesoporous silica particles with modified internal and external surfaces
文献类型：期刊论文
| 作者 | Qi, Yanxia1; Wu, Dapeng1; Wei, Junying2; Ding, Kun1; Wang, Hua1; Zhang, Yangjun2; Qian, Xiaohong2; Guan, Yafeng1 |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2010-10-01 |
| 卷号 | 398期号:4页码:1715-1722 |
| 关键词 | Interior and external modification Mesoporous silica Peptides Selective extraction |
| 产权排序 | 1;1 |
| 通讯作者 | 关亚风 |
| 英文摘要 | a new mesoporous silica material with modified external and internal surfaces (alkyl diol-vinyl-sio(2)) was prepared and applied to selectively extract low molecular weight (lmw) proteins and peptides (less than 11 kda) from biological samples. x-ray diffraction, n(2) adsorption, transmission electron microscopy, and fourier transform infrared spectroscopy were used to characterize the alkyl diol-vinyl-sio(2). this material was used to extract lmw proteins (peptides) from standard proteins, a mixture of peptides, and crude human plasma. we compared the amounts of different proteins adsorbed by alkyl diol-vinyl-sio(2) and by vinyl-sio(2) (vinyl group on the internal surface of the mesoporous silica material). the desorption recovery of alkyl diol-vinyl-sio(2) by different eluents was also investigated by using a standard protein (insulin) as a model lmw protein. the material could efficiently extract lmw proteins and peptides from a mixture of standard proteins and crude human plasma with good extraction efficiency and desorption recovery. furthermore, the alkyl diol-vinyl-sio(2) had much better extraction selectivity for lmw proteins and peptides than a commercial c(18) solid phase extraction material. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | solid-phase extraction ; low-abundance peptides ; tof ms analysis ; human plasma ; mcm-41 ; functionalization ; integration ; enrichment |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000282090000017 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/103403]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Key Lab Separat Sci Analyt Chem CAS, Dalian 116023, Peoples R China 2.Beijing Inst Radiat Med, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Yanxia,Wu, Dapeng,Wei, Junying,et al. Selective extraction of low molecular weight proteins by mesoporous silica particles with modified internal and external surfaces[J]. analytical and bioanalytical chemistry,2010,398(4):1715-1722. |
| APA | Qi, Yanxia.,Wu, Dapeng.,Wei, Junying.,Ding, Kun.,Wang, Hua.,...&Guan, Yafeng.(2010).Selective extraction of low molecular weight proteins by mesoporous silica particles with modified internal and external surfaces.analytical and bioanalytical chemistry,398(4),1715-1722. |
| MLA | Qi, Yanxia,et al."Selective extraction of low molecular weight proteins by mesoporous silica particles with modified internal and external surfaces".analytical and bioanalytical chemistry 398.4(2010):1715-1722. |
入库方式： OAI收割
来源：大连化学物理研究所
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