Visual detection of sub-femtomole DNA by a gold nanoparticle seeded homogeneous reduction assay: Toward a generalized sensitivity-enhancing strategy
文献类型：期刊论文
| 作者 | Bai, Xue1; Shao, Congying1; Han, Xiaogang1; Li, Yulin1; Guan, Yafeng2; Deng, Zhaoxiang1 |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2010-04-15 |
| 卷号 | 25期号:8页码:1984-1988 |
| 关键词 | DNA assay Gold nanoparticles Sensitivity enhancement Visual detection |
| 产权排序 | 2;5 |
| 通讯作者 | 邓兆祥 |
| 英文摘要 | gold nanoparticles (aunps) have been employed to design colorimetric visual sensing assays toward the detections of various targets including dna, based on the aggregation induced color transitions of aunps. however, the relatively high detection limit (lod 10 nm) in the case of dna detection has become a stumbling block on the road of the further development and applications of these assays. this research aims at overcoming this limit by virtue of a seeded gold reduction strategy. typically, low concentrations of 13 nm aunps modified with suitable dna probes are allowed to hybridize with a dna target to form aggregates, which are then transferred into a gold-enhancing cocktail for further depositions of more gold on the initial aunp seeds. the color of the assay thus sensitively reflects the initial aggregation status of the 13 nm aunps, which can be related to the concentrations of the dna target. this assay has a sensitivity that is at least 25-50 times improved. under still not fully optimized conditions, 0.4 fmol dna in a 2 mu l sample can be confidently detected with the ability of distinguishing a single base mutation. it does not require isolations of the 13 nm aunp aggregates during analyses and shares the advantages of a homogeneous assay, including simplicity, adaptability, convenience, and being free of interferences due to non-specific surface adsorptions. (c) 2010 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biophysics ; biotechnology & applied microbiology ; chemistry, analytical ; electrochemistry ; nanoscience & nanotechnology |
| 研究领域[WOS] | biophysics ; biotechnology & applied microbiology ; chemistry ; electrochemistry ; science & technology - other topics |
| 关键词[WOS] | wet chemical synthesis ; colorimetric detection ; aspect-ratio ; au-nanoparticles ; dnazyme ; nanorods ; aptamer ; design ; probes ; ions |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000276424200020 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/103417]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Univ Sci & Technol China, Dept Chem, CAS Key Lab Soft Matter Chem, Hefei 230026, Anhui, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Xue,Shao, Congying,Han, Xiaogang,et al. Visual detection of sub-femtomole DNA by a gold nanoparticle seeded homogeneous reduction assay: Toward a generalized sensitivity-enhancing strategy[J]. biosensors & bioelectronics,2010,25(8):1984-1988. |
| APA | Bai, Xue,Shao, Congying,Han, Xiaogang,Li, Yulin,Guan, Yafeng,&Deng, Zhaoxiang.(2010).Visual detection of sub-femtomole DNA by a gold nanoparticle seeded homogeneous reduction assay: Toward a generalized sensitivity-enhancing strategy.biosensors & bioelectronics,25(8),1984-1988. |
| MLA | Bai, Xue,et al."Visual detection of sub-femtomole DNA by a gold nanoparticle seeded homogeneous reduction assay: Toward a generalized sensitivity-enhancing strategy".biosensors & bioelectronics 25.8(2010):1984-1988. |
入库方式： OAI收割
来源：大连化学物理研究所
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