中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Precise determination, cross-recognition, and functional analysis of the double-strand origins of the rolling-circle replication plasmids in haloarchaea

文献类型:期刊论文

作者Zhou, Ligang1,2; Zhou, Meixian1; Sun, Chaomin1; Han, Jing1,2; Lu, Qiuhe1,2; Zhou, Jian1; Xiang, Hua1
刊名Journal of bacteriology
出版日期2008-08-01
卷号190期号:16页码:5710-5719
ISSN号0021-9193
DOI10.1128/jb.00596-08
通讯作者Xiang, hua(xiangh@sun.im.ac.cn)
英文摘要The precise nick site in the double-strand origin (dso) of pzmx201, a 1,668-bp rolling-circle replication (rcr) plasmid from the haloarchaeon natrinema sp. cx2021, was determined by electron microscopy and dso mapping. in this plasmid, dso nicking occurred between residues c404 and g405 within a heptanucleotide sequence (tctc/ggq located in the stem region of an imperfect hairpin structure. this nick site sequence was conserved among the haloarchaeal rcr plasmids, including pnb101, suggesting that the dso nick site might be the same for all members of this plasmid family. interestingly, the dsos of pzmx201 and pn13101 were found to be cross-recognized in rcr initiation and termination in a hybrid plasmid system. mutation analysis of the dso from pzmx201 (dso,) in this hybrid plasmid system revealed that: (i) the nucleotides in the middle of the conserved tctcggc sequence play more-important roles in the initiation and termination process; (ii) the left half of the hairpin structure is required for initiation but not for termination; and (iii) a 36-bp sequence containing tctcggc and the downstream sequence is essential and sufficient for termination. in conclusion, these haloarchaeal plasmids, with novel features that are different from the characteristics of both single-stranded dna phages and bacterial rcr plasmids, might serve as a good model for studying the evolution of rcr replicons.
WOS关键词ARCHAEON PYROCOCCUS-ABYSSI ; INITIATOR PROTEIN ; NUCLEOTIDE-SEQUENCE ; DNA-REPLICATION ; MOLECULAR CHARACTERIZATION ; HALOALKALIPHILIC ARCHAEON ; HALOBACTERIUM-VOLCANII ; BACTERIAL PLASMIDS ; MINIMAL REPLICON ; ACTIVE-SITE
WOS研究方向Microbiology
WOS类目Microbiology
语种英语
WOS记录号WOS:000258274400019
出版者AMER SOC MICROBIOLOGY
URI标识http://www.irgrid.ac.cn/handle/1471x/2389582
专题中国科学院大学
通讯作者Xiang, Hua
作者单位1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
2.Grad Univ Chinese Acad Sci, Beijing 100101, Peoples R China
推荐引用方式
GB/T 7714
Zhou, Ligang,Zhou, Meixian,Sun, Chaomin,et al. Precise determination, cross-recognition, and functional analysis of the double-strand origins of the rolling-circle replication plasmids in haloarchaea[J]. Journal of bacteriology,2008,190(16):5710-5719.
APA Zhou, Ligang.,Zhou, Meixian.,Sun, Chaomin.,Han, Jing.,Lu, Qiuhe.,...&Xiang, Hua.(2008).Precise determination, cross-recognition, and functional analysis of the double-strand origins of the rolling-circle replication plasmids in haloarchaea.Journal of bacteriology,190(16),5710-5719.
MLA Zhou, Ligang,et al."Precise determination, cross-recognition, and functional analysis of the double-strand origins of the rolling-circle replication plasmids in haloarchaea".Journal of bacteriology 190.16(2008):5710-5719.

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来源:中国科学院大学

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