Precise determination, cross-recognition, and functional analysis of the double-strand origins of the rolling-circle replication plasmids in haloarchaea
文献类型：期刊论文
| 作者 | Zhou, Ligang1,2; Zhou, Meixian1; Sun, Chaomin1; Han, Jing1,2; Lu, Qiuhe1,2; Zhou, Jian1; Xiang, Hua1 |
| 刊名 | Journal of bacteriology
 |
| 出版日期 | 2008-08-01 |
| 卷号 | 190期号:16页码:5710-5719 |
| ISSN号 | 0021-9193 |
| DOI | 10.1128/jb.00596-08 |
| 通讯作者 | Xiang, hua(xiangh@sun.im.ac.cn) |
| 英文摘要 | The precise nick site in the double-strand origin (dso) of pzmx201, a 1,668-bp rolling-circle replication (rcr) plasmid from the haloarchaeon natrinema sp. cx2021, was determined by electron microscopy and dso mapping. in this plasmid, dso nicking occurred between residues c404 and g405 within a heptanucleotide sequence (tctc/ggq located in the stem region of an imperfect hairpin structure. this nick site sequence was conserved among the haloarchaeal rcr plasmids, including pnb101, suggesting that the dso nick site might be the same for all members of this plasmid family. interestingly, the dsos of pzmx201 and pn13101 were found to be cross-recognized in rcr initiation and termination in a hybrid plasmid system. mutation analysis of the dso from pzmx201 (dso,) in this hybrid plasmid system revealed that: (i) the nucleotides in the middle of the conserved tctcggc sequence play more-important roles in the initiation and termination process; (ii) the left half of the hairpin structure is required for initiation but not for termination; and (iii) a 36-bp sequence containing tctcggc and the downstream sequence is essential and sufficient for termination. in conclusion, these haloarchaeal plasmids, with novel features that are different from the characteristics of both single-stranded dna phages and bacterial rcr plasmids, might serve as a good model for studying the evolution of rcr replicons. |
| WOS关键词 | ARCHAEON PYROCOCCUS-ABYSSI ; INITIATOR PROTEIN ; NUCLEOTIDE-SEQUENCE ; DNA-REPLICATION ; MOLECULAR CHARACTERIZATION ; HALOALKALIPHILIC ARCHAEON ; HALOBACTERIUM-VOLCANII ; BACTERIAL PLASMIDS ; MINIMAL REPLICON ; ACTIVE-SITE |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000258274400019 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2389582 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xiang, Hua |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Ligang,Zhou, Meixian,Sun, Chaomin,et al. Precise determination, cross-recognition, and functional analysis of the double-strand origins of the rolling-circle replication plasmids in haloarchaea[J]. Journal of bacteriology,2008,190(16):5710-5719. |
| APA | Zhou, Ligang.,Zhou, Meixian.,Sun, Chaomin.,Han, Jing.,Lu, Qiuhe.,...&Xiang, Hua.(2008).Precise determination, cross-recognition, and functional analysis of the double-strand origins of the rolling-circle replication plasmids in haloarchaea.Journal of bacteriology,190(16),5710-5719. |
| MLA | Zhou, Ligang,et al."Precise determination, cross-recognition, and functional analysis of the double-strand origins of the rolling-circle replication plasmids in haloarchaea".Journal of bacteriology 190.16(2008):5710-5719. |
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来源：中国科学院大学
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