Rice root architecture associated1 binds the proteasome subunit rpt4 and is degraded in a d-box and proteasome-dependent manner
文献类型：期刊论文
| 作者 | Han, Ye1,6; Cao, Hong1,6; Jiang, Jiafu1; Xu, Yunyuan1; Du, Jizhou2; Wang, Xin1; Yuan, Ming2; Wang, Zhiyong1,3,4; Xu, Zhihong1,5; Chong, Kang1,5 |
| 刊名 | Plant physiology
 |
| 出版日期 | 2008-10-01 |
| 卷号 | 148期号:2页码:843-855 |
| ISSN号 | 0032-0889 |
| DOI | 10.1104/pp.108.125294 |
| 通讯作者 | Chong, kang(chongk@ibcas.ac.cn) |
| 英文摘要 | Root growth is mainly determined by cell division and subsequent elongation in the root apical area. components regulating cell division in root meristematic cells are largely unknown. previous studies have identified rice (oryza sativa) root architecture associated1 (osraa1) as a regulator in root development. yet, the function of osraa1 at the cellular and molecular levels is unclear. here, we show that osraa1-overexpressed transgenic rice showed reduced primary root growth, increased numbers of cells in metaphase, and reduced numbers of cells in anaphase, which suggests that osraa1 is responsible for limiting root growth by inhibiting the onset of anaphase. the expression of osraa1 in fission yeast also induced metaphase arrest, which is consistent with the fact that osraa1 functions through a conserved mechanism of cell cycle regulation. moreover, a colocalization assay has shown that osraa1 is expressed predominantly at spindles during cell division. yeast two-hybrid and pull-down assays, as well as a bimolecular fluorescence complementation assay, all have revealed that osraa1 interacts with a rice homolog of regulatory particle triple-a atpase4, a component that is involved in the ubiquitin pathway. treating transgenic rice with specific inhibitors of the 26s proteasome blocked the degradation of osraa1 and increased the number of cells in metaphase. mutation of a putative ubiquitination-targeting d-box (rgsldlisl) in osraa1 interrupted the destruction of osraa1 in transgenic yeast. these results suggest that ubiquitination and proteasomic proteolysis are involved in osraa1 degradation, which is essential for the onset of anaphase, and that osraa1 may modulate root development mediated by the ubiquitin-proteasome pathway as a novel regulatory factor of the cell cycle. |
| WOS关键词 | ANAPHASE-PROMOTING COMPLEX ; POLE BODY DUPLICATION ; CELL-CYCLE REGULATION ; 26S PROTEASOME ; ORYZA-SATIVA ; SACCHAROMYCES-CEREVISIAE ; REGULATORY PARTICLE ; RADIAL ORGANIZATION ; PLANT DEVELOPMENT ; ARABIDOPSIS ROOT |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| WOS记录号 | WOS:000259810400015 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2389669 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chong, Kang |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.China Agr Univ, Dept Plant Sci, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100094, Peoples R China 3.Stanford Univ, Carnegie Inst Washington, Dept Plant Biol, Stanford, CA 94305 USA 4.Stanford Univ, Dept Biol Sci, Stanford, CA 94305 USA 5.Natl Res Ctr Plant Gene, Beijing 100093, Peoples R China 6.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Ye,Cao, Hong,Jiang, Jiafu,et al. Rice root architecture associated1 binds the proteasome subunit rpt4 and is degraded in a d-box and proteasome-dependent manner[J]. Plant physiology,2008,148(2):843-855. |
| APA | Han, Ye.,Cao, Hong.,Jiang, Jiafu.,Xu, Yunyuan.,Du, Jizhou.,...&Chong, Kang.(2008).Rice root architecture associated1 binds the proteasome subunit rpt4 and is degraded in a d-box and proteasome-dependent manner.Plant physiology,148(2),843-855. |
| MLA | Han, Ye,et al."Rice root architecture associated1 binds the proteasome subunit rpt4 and is degraded in a d-box and proteasome-dependent manner".Plant physiology 148.2(2008):843-855. |
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来源：中国科学院大学
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