Microvalve and micropump controlled shuttle flow microfluidic device for rapid DNA hybridization
文献类型：期刊论文
| 作者 | Huang, Shuqiang1,2; Li, Chunyu1,2; Lin, Bingcheng1; Qin, Jianhua1 |
| 刊名 | lab on a chip
 |
| 出版日期 | 2010 |
| 卷号 | 10期号:21页码:2925-2931 |
| 产权排序 | 1;1 |
| 通讯作者 | 林炳承 ; 秦建华 |
| 英文摘要 | we present a novel microfluidic device integrated with microvalves and micropumps for rapid dna hybridization using shuttle flow. the device is composed of 48 hybridization units containing 48 microvalves and 96 micropumps for the automation of shuttle flow. we used four serotypes of dengue virus genes (18mer) to demonstrate that the automatic shuttle flow shortened the hybridization time to 90 s, reduced sample consumption to 1 mu l and lowered detection limit to 100 pm (100 amol in a 1 mu l sample). moreover, we applied this device to realize single base discrimination and analyze 48 samples containing different dna targets, simultaneously. for kinetic measurements of nucleotide hybridization, on-line monitoring of the processes was carried out. this rapid hybridization device has the ability for accommodating the entire hybridization process (i.e., injection, hybridization, washing, detection, signal acquisition) in an automated and high-throughput fashion. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, multidisciplinary ; nanoscience & nanotechnology |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry ; science & technology - other topics |
| 关键词[WOS] | gene-expression patterns ; microarray hybridization ; poly(methyl methacrylate) ; heteroduplex analysis ; gel-electrophoresis ; chip ; discrimination ; immobilization ; polymorphisms ; diagnostics |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000282808700015 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/103437]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Shuqiang,Li, Chunyu,Lin, Bingcheng,et al. Microvalve and micropump controlled shuttle flow microfluidic device for rapid DNA hybridization[J]. lab on a chip,2010,10(21):2925-2931. |
| APA | Huang, Shuqiang,Li, Chunyu,Lin, Bingcheng,&Qin, Jianhua.(2010).Microvalve and micropump controlled shuttle flow microfluidic device for rapid DNA hybridization.lab on a chip,10(21),2925-2931. |
| MLA | Huang, Shuqiang,et al."Microvalve and micropump controlled shuttle flow microfluidic device for rapid DNA hybridization".lab on a chip 10.21(2010):2925-2931. |
入库方式： OAI收割
来源：大连化学物理研究所
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