Wax-bonding 3D microfluidic chips
文献类型：期刊论文
| 作者 | Gong, Xiuqing2; Yi, Xin2; Xiao, Kang3; Li, Shunbo2; Kodzius, Rimantas4; Qin, Jianhua1; Wen, Weijia2,4 |
| 刊名 | lab on a chip
 |
| 出版日期 | 2010 |
| 卷号 | 10期号:19页码:2622-2627 |
| 产权排序 | 4;6 |
| 通讯作者 | weijia wen |
| 英文摘要 | we report a simple, low-cost and detachable microfluidic chip incorporating easily accessible paper, glass slides or other polymer films as the chip materials along with adhesive wax as the recycling bonding material. we use a laser to cut through the paper or film to form patterns and then sandwich the paper and film between glass sheets or polymer membranes. the hot-melt adhesive wax can realize bridge bonding between various materials, for example, paper, polymethylmethacrylate (pmma) film, glass sheets, or metal plate. the bonding process is reversible and the wax is reusable through a melting and cooling process. with this process, a three-dimensional (3d) microfluidic chip is achievable by vacuating and venting the chip in a hot-water bath. to study the biocompatibility and applicability of the wax-based microfluidic chip, we tested the pcr compatibility with the chip materials first. then we applied the wax-paper based microfluidic chip to hela cell electroporation (ep). subsequently, a prototype of a 5-layer 3d chip was fabricated by multilayer wax bonding. to check the sealing ability and the durability of the chip, green fluorescence protein (gfp) recombinant escherichia coli (e. coli) bacteria were cultured, with which the chemotaxis of e. coli was studied in order to determine the influence of antibiotic ciprofloxacin concentration on the e. coli migration. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, multidisciplinary ; nanoscience & nanotechnology |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry ; science & technology - other topics |
| 关键词[WOS] | paper-based microfluidics ; electroporation chip ; low-cost ; devices ; fabrication ; systems ; poly(dimethylsiloxane) ; cells ; visualization ; pdms |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000281614900021 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/103441]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Beijing 11603, Peoples R China 2.Hong Kong Univ Sci & Technol, Dept Phys, Kowloon, Hong Kong, Peoples R China 3.Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China 4.Hong Kong Univ Sci & Technol, KAUST HKUST Micro Nanofluid Joint Lab, Kowloon, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gong, Xiuqing,Yi, Xin,Xiao, Kang,et al. Wax-bonding 3D microfluidic chips[J]. lab on a chip,2010,10(19):2622-2627. |
| APA | Gong, Xiuqing.,Yi, Xin.,Xiao, Kang.,Li, Shunbo.,Kodzius, Rimantas.,...&Wen, Weijia.(2010).Wax-bonding 3D microfluidic chips.lab on a chip,10(19),2622-2627. |
| MLA | Gong, Xiuqing,et al."Wax-bonding 3D microfluidic chips".lab on a chip 10.19(2010):2622-2627. |
入库方式： OAI收割
来源：大连化学物理研究所
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