Cloning and expression of an actin gene in the haemocytes of pearl oyster (pinctada fucata, gould 1850)
文献类型：期刊论文
| 作者 | Wang, Zhongliang1,2,3,4,5; Wu, Zaohe1,4,5; Jian, Jichang1,4,5; Lu, Yishan1,4,5 |
| 刊名 | Marine genomics
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 1期号:2页码:63-67 |
| 关键词 | Homological cloning Race Bacterial challenge Real-time pcr |
| ISSN号 | 1874-7787 |
| DOI | 10.1016/j.margen.2008.06.004 |
| 通讯作者 | Wu, zaohe(wuzh@gdou.edu.cn) |
| 英文摘要 | An actin gene (designated pfact1) of pearl oyster, pinctada fucata, was cloned from haemocytes by the techniques of homological cloning and rapid amplification of cdna ends (race). the full length of pfact1 cdna was 1608 bp in length, having a 51 untranslated region (utr) of 82 bp, a 3' utr of 395 bp, and an open reading frame (orf) of 1131 bp encoding a polypeptide of 376 amino acids with a predicted molecular weight of 41.76 kda and an estimated isoelectric point of 5.29. sequence analysis revealed that pfact1 shared high similarity with other actins and was more closely related to vertebrate cytoplastic actins than muscle types. phylogenetic analysis indicated that molluscan actins could also be generally grouped into two classes: muscle type and cytoplasmic type, although both are similar to vertebrate cytoplastic actins. fluorescent real-time quantitative rt-pcr was used to examine the expression level of pfact1 in haemocytes of p. fucata after the challenge of vibrio alginolyticus, and results showed that pfact1 exhibited stable expression in all time points, indicating that pfact1 could be a suitable internal control for gene expression analysis in haemocytes of p. fucata. (c) 2008 elsevier b.v. all rights reserved. |
| WOS研究方向 | Genetics & Heredity ; Marine & Freshwater Biology |
| WOS类目 | Genetics & Heredity ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000207472700004 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2389726 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wu, Zaohe |
| 作者单位 | 1.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524025, Guangdong Prov, Peoples R China 2.Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.Guangdong Key Lab Pathogen Biol & Epidemiol Aquat, Zhanjiang, Peoples R China 5.Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Zhongliang,Wu, Zaohe,Jian, Jichang,et al. Cloning and expression of an actin gene in the haemocytes of pearl oyster (pinctada fucata, gould 1850)[J]. Marine genomics,2008,1(2):63-67. |
| APA | Wang, Zhongliang,Wu, Zaohe,Jian, Jichang,&Lu, Yishan.(2008).Cloning and expression of an actin gene in the haemocytes of pearl oyster (pinctada fucata, gould 1850).Marine genomics,1(2),63-67. |
| MLA | Wang, Zhongliang,et al."Cloning and expression of an actin gene in the haemocytes of pearl oyster (pinctada fucata, gould 1850)".Marine genomics 1.2(2008):63-67. |
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来源：中国科学院大学
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