A novel c2-domain phospholipid-binding protein, ospbp1, is required for pollen fertility in rice
文献类型：期刊论文
| 作者 | Yang, Wen-Qiang1,2,3; Lai, Ying1,2,3; Li, Mei-Na1,2,3; Xu, Wen-Ying1,2; Xue, Yong-Biao1,2 |
| 刊名 | Molecular plant
 |
| 出版日期 | 2008-09-01 |
| 卷号 | 1期号:5页码:770-785 |
| 关键词 | Rice (oryza sativa l.) Pollination Pollen fertility Ospbp1 C2-domain Phospholipid |
| ISSN号 | 1674-2052 |
| DOI | 10.1093/mp/ssn035 |
| 通讯作者 | Xue, yong-biao(ybxue@genetics.ac.cn) |
| 英文摘要 | Pollen fertility is a crucial factor for successful pollination and essential for seed formation. recent studies have suggested that a diverse range of internal and external factors, signaling components and their related pathways are likely involved in pollen fertility. here, we report a single c2-domain containing protein, ospbp1, initially identified through cdna microarray analysis. ospbp1 is a single copy gene and preferentially expressed in pistil and pollen but downregulated by pollination. ospbp1 had a calcium concentration-dependent phospholipid-binding activity and was localized mainly in cytoplasm and nucleus, but translocated onto the plasma membrane in response to an intracellular ca2+ increase. pollen grains of antisense ospbp1 transgenic lines were largely nonviable, germinated poorly in vitro and of low fertility. ospbp1 protein was localized in a region peripheral to pollen wall and vesicles of elongating pollen tube, and its repressed expression reduced substantially this association and led to alteration of microfilament polymerization during pollen germination. taken together, these results indicate that ospbp1 is a novel functional c2-domain phospholipids-binding protein that is required for pollen fertility likely by regulating ca2+ and phospholipid signaling pathways. |
| WOS关键词 | TUBE GROWTH ; C2 DOMAIN ; MEMBRANE-BINDING ; ZINC-FINGER ; KINASE-C ; ENDOPLASMIC-RETICULUM ; SELF-INCOMPATIBILITY ; WAX BIOSYNTHESIS ; PLANT-GROWTH ; ARABIDOPSIS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000259104500006 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2390283 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xue, Yong-Biao |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100190, Peoples R China 2.Natl Ctr Plant Gene Res, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Wen-Qiang,Lai, Ying,Li, Mei-Na,et al. A novel c2-domain phospholipid-binding protein, ospbp1, is required for pollen fertility in rice[J]. Molecular plant,2008,1(5):770-785. |
| APA | Yang, Wen-Qiang,Lai, Ying,Li, Mei-Na,Xu, Wen-Ying,&Xue, Yong-Biao.(2008).A novel c2-domain phospholipid-binding protein, ospbp1, is required for pollen fertility in rice.Molecular plant,1(5),770-785. |
| MLA | Yang, Wen-Qiang,et al."A novel c2-domain phospholipid-binding protein, ospbp1, is required for pollen fertility in rice".Molecular plant 1.5(2008):770-785. |
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来源：中国科学院大学
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