中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
A novel c2-domain phospholipid-binding protein, ospbp1, is required for pollen fertility in rice

文献类型:期刊论文

作者Yang, Wen-Qiang1,2,3; Lai, Ying1,2,3; Li, Mei-Na1,2,3; Xu, Wen-Ying1,2; Xue, Yong-Biao1,2
刊名Molecular plant
出版日期2008-09-01
卷号1期号:5页码:770-785
关键词Rice (oryza sativa l.) Pollination Pollen fertility Ospbp1 C2-domain Phospholipid
ISSN号1674-2052
DOI10.1093/mp/ssn035
通讯作者Xue, yong-biao(ybxue@genetics.ac.cn)
英文摘要Pollen fertility is a crucial factor for successful pollination and essential for seed formation. recent studies have suggested that a diverse range of internal and external factors, signaling components and their related pathways are likely involved in pollen fertility. here, we report a single c2-domain containing protein, ospbp1, initially identified through cdna microarray analysis. ospbp1 is a single copy gene and preferentially expressed in pistil and pollen but downregulated by pollination. ospbp1 had a calcium concentration-dependent phospholipid-binding activity and was localized mainly in cytoplasm and nucleus, but translocated onto the plasma membrane in response to an intracellular ca2+ increase. pollen grains of antisense ospbp1 transgenic lines were largely nonviable, germinated poorly in vitro and of low fertility. ospbp1 protein was localized in a region peripheral to pollen wall and vesicles of elongating pollen tube, and its repressed expression reduced substantially this association and led to alteration of microfilament polymerization during pollen germination. taken together, these results indicate that ospbp1 is a novel functional c2-domain phospholipids-binding protein that is required for pollen fertility likely by regulating ca2+ and phospholipid signaling pathways.
WOS关键词TUBE GROWTH ; C2 DOMAIN ; MEMBRANE-BINDING ; ZINC-FINGER ; KINASE-C ; ENDOPLASMIC-RETICULUM ; SELF-INCOMPATIBILITY ; WAX BIOSYNTHESIS ; PLANT-GROWTH ; ARABIDOPSIS
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences
WOS类目Biochemistry & Molecular Biology ; Plant Sciences
语种英语
WOS记录号WOS:000259104500006
出版者OXFORD UNIV PRESS
URI标识http://www.irgrid.ac.cn/handle/1471x/2390283
专题中国科学院大学
通讯作者Xue, Yong-Biao
作者单位1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100190, Peoples R China
2.Natl Ctr Plant Gene Res, Beijing 100190, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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GB/T 7714
Yang, Wen-Qiang,Lai, Ying,Li, Mei-Na,et al. A novel c2-domain phospholipid-binding protein, ospbp1, is required for pollen fertility in rice[J]. Molecular plant,2008,1(5):770-785.
APA Yang, Wen-Qiang,Lai, Ying,Li, Mei-Na,Xu, Wen-Ying,&Xue, Yong-Biao.(2008).A novel c2-domain phospholipid-binding protein, ospbp1, is required for pollen fertility in rice.Molecular plant,1(5),770-785.
MLA Yang, Wen-Qiang,et al."A novel c2-domain phospholipid-binding protein, ospbp1, is required for pollen fertility in rice".Molecular plant 1.5(2008):770-785.

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来源:中国科学院大学

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