Macroporous molecularly imprinted monolithic polymer columns for protein recognition by liquid chromatography
文献类型：期刊论文
| 作者 | Liu, Jinxiang1,2; Deng, Qiliang3; Yang, Kaiguang1; Zhang, Lihua1; Liang, Zhen1; Zhang, Yukui1 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2010-09-01 |
| 卷号 | 33期号:17-18页码:2757-2761 |
| 关键词 | HPLC Molecular imprinting Polyacrylamide monolith Protein recognition |
| 通讯作者 | 张丽华 |
| 产权排序 | 1;1 |
| 英文摘要 | macroporous cytochrome c (cyc)-imprinted monolithic polyarylamide columns were prepared, and applied for the template protein recognition by hplc. with cyc (18.8 mg) as template, the imprinted monolithic materials were in situ polymerized in an hplc column tube, with methacrylamide (450 mg), methacrylic acid (15.8 mg), piperazine diacrylamide (720 mg) and ammonium sulfate (390 mg) dissolved in 5 ml of phosphate buffer (ph 7.4), initiated by ammonium persulfate and temed. after the reaction, cyc was removed with acetic acid (10%, v/v) containing 10% w/v sds. the non-imprinted monolithic column was prepared under the same procedure except without cyc. retention of cyc and its competitive protein, lysozyme (lys), on molecular-imprinting polymer (mip) and non-imprinted polymer columns was studied. when the ph value of mobile phase was 4.0, on mip column, the retention factors of cyc and lys were 2.0 and 1.3, respectively. however, those on non-imprinted polymer column were very similar, both as 1.1. even in competitive environment, a mixture of cyc and lys could be separated on mip column under gradient elution, with resolution as 1.2. these results indicate that protein-imprinted monolithic polymer columns could offer obvious affinity and specific recognition to the template protein. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | stationary phases ; antibodies ; delivery ; blood |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000282612900025 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/103559]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.China Acad Sci, Grad Univ, Beijing, Peoples R China 3.Tianjin Univ Sci & Technol, Coll Sci, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jinxiang,Deng, Qiliang,Yang, Kaiguang,et al. Macroporous molecularly imprinted monolithic polymer columns for protein recognition by liquid chromatography[J]. journal of separation science,2010,33(17-18):2757-2761. |
| APA | Liu, Jinxiang,Deng, Qiliang,Yang, Kaiguang,Zhang, Lihua,Liang, Zhen,&Zhang, Yukui.(2010).Macroporous molecularly imprinted monolithic polymer columns for protein recognition by liquid chromatography.journal of separation science,33(17-18),2757-2761. |
| MLA | Liu, Jinxiang,et al."Macroporous molecularly imprinted monolithic polymer columns for protein recognition by liquid chromatography".journal of separation science 33.17-18(2010):2757-2761. |
入库方式： OAI收割
来源：大连化学物理研究所
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