中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Protein separation using free-flow electrophoresis microchip etched in a single step

文献类型:期刊论文

作者Wang, Pingli1,2; Zhang, Lihua1; Shan, Yichu1; Cong, Yongzheng1,2; Liang, Yu1,2; Han, Bin1,3; Liang, Zhen1; Zhang, Yukui1
刊名journal of separation science
出版日期2010-07-01
卷号33期号:13页码:2039-2044
关键词Fluorescence image Free-flow electrophoresis Microchip One-step etching Protein separation
产权排序1;1
通讯作者张丽华
英文摘要a one-step etching method was developed to fabricate glass free-flow electrophoresis microchips with a rectangle separation microchamber (42 mm-long, 23 mm-wide and 28 mu m-deep), in which two glass bridges (0.5 mm-wide) were made simultaneously to prevent bubbles formed by electrolysis near the pt electrode from entering the separation chamber. by microchip free-flow zone electrophoresis, with 200 v voltage applied, the baseline separation of three fitc labeled proteins, ribonuclease b, myoglobin and beta-lactoglobulin, was achieved, with resolution over 1.78. furthermore, with 2.5 mivl na(2)so(4) added into the electrode buffer to form higher electrical field strength across separation microchamber than electrode compartments, similar resolution of samples was achieved with the applied voltage decreased to 75 v, which could obviously decrease joule heat during continuous separation. all these results demonstrate that the free-flow electrophoresis microchip fabricated by one-step etching method is suitable for the continuous separation of proteins, which might become an effective pre-fractionation method for proteome study.
WOS标题词science & technology ; physical sciences
类目[WOS]chemistry, analytical
研究领域[WOS]chemistry
关键词[WOS]mass-spectrometry ; device ; chip ; proteomics ; resolution ; speed
收录类别SCI
原文出处false
语种英语
WOS记录号WOS:000280163900022
公开日期2010-11-30
源URL[http://159.226.238.44/handle/321008/103567]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Wang, Pingli,Zhang, Lihua,Shan, Yichu,et al. Protein separation using free-flow electrophoresis microchip etched in a single step[J]. journal of separation science,2010,33(13):2039-2044.
APA Wang, Pingli.,Zhang, Lihua.,Shan, Yichu.,Cong, Yongzheng.,Liang, Yu.,...&Zhang, Yukui.(2010).Protein separation using free-flow electrophoresis microchip etched in a single step.journal of separation science,33(13),2039-2044.
MLA Wang, Pingli,et al."Protein separation using free-flow electrophoresis microchip etched in a single step".journal of separation science 33.13(2010):2039-2044.

入库方式: OAI收割

来源:大连化学物理研究所

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