Protein separation using free-flow electrophoresis microchip etched in a single step
文献类型：期刊论文
| 作者 | Wang, Pingli1,2; Zhang, Lihua1; Shan, Yichu1; Cong, Yongzheng1,2; Liang, Yu1,2; Han, Bin1,3; Liang, Zhen1; Zhang, Yukui1 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2010-07-01 |
| 卷号 | 33期号:13页码:2039-2044 |
| 关键词 | Fluorescence image Free-flow electrophoresis Microchip One-step etching Protein separation |
| 产权排序 | 1;1 |
| 通讯作者 | 张丽华 |
| 英文摘要 | a one-step etching method was developed to fabricate glass free-flow electrophoresis microchips with a rectangle separation microchamber (42 mm-long, 23 mm-wide and 28 mu m-deep), in which two glass bridges (0.5 mm-wide) were made simultaneously to prevent bubbles formed by electrolysis near the pt electrode from entering the separation chamber. by microchip free-flow zone electrophoresis, with 200 v voltage applied, the baseline separation of three fitc labeled proteins, ribonuclease b, myoglobin and beta-lactoglobulin, was achieved, with resolution over 1.78. furthermore, with 2.5 mivl na(2)so(4) added into the electrode buffer to form higher electrical field strength across separation microchamber than electrode compartments, similar resolution of samples was achieved with the applied voltage decreased to 75 v, which could obviously decrease joule heat during continuous separation. all these results demonstrate that the free-flow electrophoresis microchip fabricated by one-step etching method is suitable for the continuous separation of proteins, which might become an effective pre-fractionation method for proteome study. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | mass-spectrometry ; device ; chip ; proteomics ; resolution ; speed |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000280163900022 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/103567]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Pingli,Zhang, Lihua,Shan, Yichu,et al. Protein separation using free-flow electrophoresis microchip etched in a single step[J]. journal of separation science,2010,33(13):2039-2044. |
| APA | Wang, Pingli.,Zhang, Lihua.,Shan, Yichu.,Cong, Yongzheng.,Liang, Yu.,...&Zhang, Yukui.(2010).Protein separation using free-flow electrophoresis microchip etched in a single step.journal of separation science,33(13),2039-2044. |
| MLA | Wang, Pingli,et al."Protein separation using free-flow electrophoresis microchip etched in a single step".journal of separation science 33.13(2010):2039-2044. |
入库方式： OAI收割
来源：大连化学物理研究所
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