中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Protein imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers

文献类型:期刊论文

作者Zhang, Wei1; Qin, Lei1; Chen, Run-Run1; He, Xi-Wen1; Li, Wen-You1; Zhang, Yu-Kui1,2
刊名applied surface science
出版日期2010-02-15
卷号256期号:9页码:3000-3005
关键词Surface molecular imprinting beta-Cyclodextrin Bovine hemoglobin Recognition
产权排序6;2
通讯作者李文友
英文摘要a novel protein imprinted polymer was prepared using acryloyl-beta-cyclodextrin (beta-cd) and acrylamide as monomers on the surface of silica gel. the bovine hemoglobin was used as template and beta-cd was allowed to self-assemble with the template protein through hydrogen bonding and hydrophobic interaction. polymerization was carried out in the presence of acrylamide as an assistant monomer, which resulted in a novel protein imprinted polymer. after removing the template, imprinted cavities with the shape and spatial distribution of functional groups were formed. bovine serum albumin (bsa) cytochrome c (cyt) and lysozyme (lyz) were employed as non-template proteins to test the imprinting effect and the specific binding of bovine hemoglobin to the polymer. the results of the adsorption experiments indicated that such protein imprinted polymer, which was synthesized with beta-cd and acrylamide as monomers, could selectively recognize the template protein. (c) 2009 elsevier b. v. all rights reserved.
WOS标题词science & technology ; physical sciences ; technology
类目[WOS]chemistry, physical ; materials science, coatings & films ; physics, applied ; physics, condensed matter
研究领域[WOS]chemistry ; materials science ; physics
关键词[WOS]bovine serum-albumin ; submicrometer particles ; selective recognition ; aqueous-media ; surface ; gel ; antibodies ; separation ; peptides ; beads
收录类别SCI
原文出处false
语种英语
WOS记录号WOS:000274153800055
公开日期2010-11-30
源URL[http://159.226.238.44/handle/321008/103577]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
作者单位1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Wei,Qin, Lei,Chen, Run-Run,et al. Protein imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers[J]. applied surface science,2010,256(9):3000-3005.
APA Zhang, Wei,Qin, Lei,Chen, Run-Run,He, Xi-Wen,Li, Wen-You,&Zhang, Yu-Kui.(2010).Protein imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers.applied surface science,256(9),3000-3005.
MLA Zhang, Wei,et al."Protein imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers".applied surface science 256.9(2010):3000-3005.

入库方式: OAI收割

来源:大连化学物理研究所

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