Delayed resistance to cucumber mosaic virus mediated by 3'utr-derived hairpin rna
文献类型：期刊论文
| 作者 | Duan ChengGuo1,2,3; Wang ChunHan1,2,4; Guo HuiShan1,2 |
| 刊名 | Chinese science bulletin
 |
| 出版日期 | 2008-11-01 |
| 卷号 | 53期号:21页码:3301-3310 |
| 关键词 | Cmv 3'utr Rna silencing Sirna Viral resistance |
| ISSN号 | 1001-6538 |
| DOI | 10.1007/s11434-008-0440-0 |
| 通讯作者 | Guo huishan(guohs@im.ac.cn) |
| 英文摘要 | Rna silencing has been shown to function in the plant antivirus defense response, leading to viral rna degradation induced by vsirna-containing risc cleavage activity. cucumber mosaic virus (cmv) 3'utr sequences share a high conservation of nucleotide sequence and secondary structures that are important for cmv replication. here, in an attempt to simultaneously target the multiple genomic and subgenomic rnas of cmv for degradation, cmv 3'utr were used to design hairpin rna (hprna) to transform tobacco (xanthi. nc) so as to constitutively produce viral sirnas. most of the transgenic plants expressing cmv q strain (q-cmv, subgroup ii strain) rna3 3'utr-derived hprna showed delayed resistance to q-cmv infection and exhibited recovery phenotypes. compared with q-cmv-inoculated leaves, the upper leaves showed weak or no disease symptoms and a reduced accumulation level of viral rnas. together with transient assays, our results indicate that the 3'utr-derived sirnas were biologically active in targeting viral rna for degradation. recovery resistance in transgenic plants was also observed against subgroup ib strain sd-cmv infection, indicating a broad-spectrum anti-cmv effect of the 3'utr-based antiviral silencing. northern blot assays indicated that there was no strong correlation between the degree of resistance and the accumulation level of 3'utr-derived sirnas, suggesting that to target a highly structured rna, such as the cmv 3'utr, the quantity of sirnas may not be the only determinant of silencing efficiency. target rna secondary structures may also affect target accessibility, sirna-containing risc-target recognition and the consequent antiviral effect. |
| WOS关键词 | DOUBLE-STRANDED-RNA ; PLANT-VIRUSES ; SILENCING MECHANISM ; TRANSGENIC PLANTS ; GENE-EXPRESSION ; VIRAL RNAS ; POLYMERASE ; INDUCTION ; DEFENSE ; TOBACCO |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000260510700008 |
| 出版者 | SCIENCE PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2390402 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Guo HuiShan |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, Natl Ctr Plant Gene Res Beijing, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Biophys, Technol Transfer & Liaison Off, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan ChengGuo,Wang ChunHan,Guo HuiShan. Delayed resistance to cucumber mosaic virus mediated by 3'utr-derived hairpin rna[J]. Chinese science bulletin,2008,53(21):3301-3310. |
| APA | Duan ChengGuo,Wang ChunHan,&Guo HuiShan.(2008).Delayed resistance to cucumber mosaic virus mediated by 3'utr-derived hairpin rna.Chinese science bulletin,53(21),3301-3310. |
| MLA | Duan ChengGuo,et al."Delayed resistance to cucumber mosaic virus mediated by 3'utr-derived hairpin rna".Chinese science bulletin 53.21(2008):3301-3310. |
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来源：中国科学院大学
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