Magnetic Silica-Coated Sub-Microspheres with Immobilized Metal Ions for the Selective Removal of Bovine Hemoglobin from Bovine Blood
文献类型：期刊论文
| 作者 | Zhang, Min1; Cheng, Dan1; He, Xiwen1; Chen, Langxing1; Zhang, Yukui1,2 |
| 刊名 | chemistry-an asian journal
 |
| 出版日期 | 2010 |
| 卷号 | 5期号:6页码:1332-1340 |
| 关键词 | bovine hemoglobin immobilization magnetic properties removal silanes |
| 产权排序 | 5;2 |
| 通讯作者 | 陈朗星 |
| 英文摘要 | magnetic silica-coated magnetite (fe(3)o(4)) sub-microspheres with immobilized metal-affinity ligands are prepared for protein adsorption. first, magnetite sub-microspheres were synthesized by a hydrothermal method. then silica was coated on the surface of fe(3)o(4) particles using a sol gel method to obtain magnetic silica sub-microspheres with core-shell morphology. next, the trichloro(4-chloromethyl-phenyl) silane was immobilized on them, reacted with iminodiacetic acid (ida), and charged with cu(2+). the obtained magnetic silica sub-microspheres with immobilized cu(2+) were applied for the absorption of bovine hemoglobin (bhb) and the removal of bhb from bovine blood. the size, morphology, and magnetic properties of the resulting magnetic micro(nano) spheres were investigated by using scanning microscopy (sem), transmission electron microscopy (tem), x-ray diffraction (xrd), and a vibrating sample magnetometer (vsm). the measurements showed that the magnetic sub-micro-spheres are spherical in shape, very uniform in size with a core-shell, and are almost superparamagnetic. the saturation magnetization of silica-coated magnetite (fe(3)o(4)) sub-microspheres reached about 33 emu g(-1). protein adsorption results showed that the sub-microspheres had a high adsorption capacity for bhb (418.6 mg g(-1)), low nonspecific adsorption, and good removal of bhb from bovine blood. this opens a novel route for future applications in removing abundant proteins in proteomic analysis. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | tof-ms analysis ; ionization mass-spectrometry ; low-concentration peptides ; histidine-tagged proteins ; iron-oxide nanoparticles ; facile synthesis ; affinity-chromatography ; polymer nanoparticles ; enrichment ; shell |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000278840200011 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/103583]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Min,Cheng, Dan,He, Xiwen,et al. Magnetic Silica-Coated Sub-Microspheres with Immobilized Metal Ions for the Selective Removal of Bovine Hemoglobin from Bovine Blood[J]. chemistry-an asian journal,2010,5(6):1332-1340. |
| APA | Zhang, Min,Cheng, Dan,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2010).Magnetic Silica-Coated Sub-Microspheres with Immobilized Metal Ions for the Selective Removal of Bovine Hemoglobin from Bovine Blood.chemistry-an asian journal,5(6),1332-1340. |
| MLA | Zhang, Min,et al."Magnetic Silica-Coated Sub-Microspheres with Immobilized Metal Ions for the Selective Removal of Bovine Hemoglobin from Bovine Blood".chemistry-an asian journal 5.6(2010):1332-1340. |
入库方式： OAI收割
来源：大连化学物理研究所
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