Development of Identification for Post-Translational Modifications in Histones by Mass Spectrometry Based Proteomics
文献类型：期刊论文
| 作者 | Chen Ying1; Zhang Kai1; He Xiwen1; Zhang Yukui1,2 |
| 刊名 | progress in chemistry
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 22期号:4页码:713-719 |
| 关键词 | histone post-translational modifications (PTMs) mass spectrometry high prerormance liquid chromatography (HPLC) |
| 产权排序 | 4;2 |
| 通讯作者 | zhang kai |
| 英文摘要 | in eukaryotic cells, chromosomal dna is packaged into a compact structure, chromatin, with the help of four core histones. the fundamental repeating unit of chromatin is the nucleosome, which contains an octamer of core histones, around 147 base pairs of dna are wrapped. post-translational modifications (ptms) in histones are generally considered to be a major group of epigenetic marks. accordingly, because ptms alter the properties of histones, hence affect chromatin structure and function and regulate the gene expression. therefore identification of histone ptms is the key to discover histone codes. at present, mass spectrometry has become a powerful analysis tool. in this paper we introduced the novel development of the strategies based on mass spectrometry ("bottom-up" and "top-down") in mapping histone ptms, fragment dissociation technologies such as cid, ecd and etd. we also reported the application of mass spectrometry in addressing histone ptms sites, identifying histone variants and quantifying differential expression of these ptms in cells. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | electron-transfer dissociation ; h3 lysine-56 acetylation ; top-down proteomics ; h1 isoforms ; cell-cycle ; chicken erythrocytes ; dynamic changes ; global changes ; core histones ; amino-acids |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000276929300018 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/103585]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen Ying,Zhang Kai,He Xiwen,et al. Development of Identification for Post-Translational Modifications in Histones by Mass Spectrometry Based Proteomics[J]. progress in chemistry,2010,22(4):713-719. |
| APA | Chen Ying,Zhang Kai,He Xiwen,&Zhang Yukui.(2010).Development of Identification for Post-Translational Modifications in Histones by Mass Spectrometry Based Proteomics.progress in chemistry,22(4),713-719. |
| MLA | Chen Ying,et al."Development of Identification for Post-Translational Modifications in Histones by Mass Spectrometry Based Proteomics".progress in chemistry 22.4(2010):713-719. |
入库方式： OAI收割
来源：大连化学物理研究所
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