中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Effect of grazing intensities on the activity and community structure of methane-oxidizing bacteria of grassland soil in inner mongolia

文献类型:期刊论文

作者Zhou, Xiao-Qi2,3; Wang, Yan-Fen1,2; Huang, Xiang-Zhong2; Tian, Jian-Qing2; Hao, Yan-Bin2
刊名Nutrient cycling in agroecosystems
出版日期2008-02-01
卷号80期号:2页码:145-152
关键词Grazing intensities Methane flux Dgge Methanotroph Grassland
ISSN号1385-1314
DOI10.1007/s10705-007-9127-1
通讯作者Wang, yan-fen(yfwang@gucas.ac.cn)
英文摘要The effects of different grazing intensities on in situ methane flux and the structure and diversity of the methanotrophic community are measured in the typical grassland of inner mongolia. four grazing intensity sites founded in 1989, control (ck), low-intensity grazing (lg), middle-intensity grazing (mg) and heavy-intensity grazing (hg), were selected. group-specific pcr-dgge (polymerase chain reaction-denaturing gradient gel electrophoresis) of 16s rrna genes for the type i and type ii methanotrophs was used to characterize the composition of the methanotrophic community. dgge patterns were further analyzed using the method of the shannon-wiener index h and non-metric multi-dimensional scaling (mds). the results showed that there were no significant differences in methane flux among different sites, yet methanotrophic communities showed significant differences. mds analysis showed that type i methanotroph community composition at the ck site were significantly different from the three other sites. for type ii methanotrophic community composition, it was similar between ck and hg site, and between lg and mg site, while that at the former two sites were significantly different from latter two ones. additionally shannon indices of type ii methanotrophs were higher at the lg and mg sites than two other sites. though grazing intensities had an impact on the structure of the methanotrophic community, management-induced changes in the structure of methanotrophic community did not reflect methane consumption capacity across sites. these results suggest that methane consumption is a complex process in soil, and we should be cautious when speculating on the change of methane consumption rates based on a change of methanotrophic community structure.
WOS关键词GRADIENT GEL-ELECTROPHORESIS ; 16S RIBOSOMAL-RNA ; ATMOSPHERIC METHANE ; METHANOTROPHIC BACTERIA ; CH4 OXIDATION ; FOREST SOIL ; NITROGEN ; IMPACT ; FLUXES ; DIVERSITY
WOS研究方向Agriculture
WOS类目Soil Science
语种英语
WOS记录号WOS:000252639200004
出版者SPRINGER
URI标识http://www.irgrid.ac.cn/handle/1471x/2390428
专题中国科学院大学
通讯作者Wang, Yan-Fen
作者单位1.Grad Univ, Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China
2.Grad Univ, Chinese Acad Sci, Dept Biol, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
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GB/T 7714
Zhou, Xiao-Qi,Wang, Yan-Fen,Huang, Xiang-Zhong,et al. Effect of grazing intensities on the activity and community structure of methane-oxidizing bacteria of grassland soil in inner mongolia[J]. Nutrient cycling in agroecosystems,2008,80(2):145-152.
APA Zhou, Xiao-Qi,Wang, Yan-Fen,Huang, Xiang-Zhong,Tian, Jian-Qing,&Hao, Yan-Bin.(2008).Effect of grazing intensities on the activity and community structure of methane-oxidizing bacteria of grassland soil in inner mongolia.Nutrient cycling in agroecosystems,80(2),145-152.
MLA Zhou, Xiao-Qi,et al."Effect of grazing intensities on the activity and community structure of methane-oxidizing bacteria of grassland soil in inner mongolia".Nutrient cycling in agroecosystems 80.2(2008):145-152.

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来源:中国科学院大学

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