Characterization and application of monoclonal antibodies specific to west nile virus envelope protein
文献类型：期刊论文
| 作者 | Liu, June1,2,7; Liu, Bohua3; Cao, Zhen4; Inoue, Shingo5; Morita, Kouichi5; Tian, Kegong4; Zhu, Qingyu3; Gao, George F.1,2,6 |
| 刊名 | Journal of virological methods
 |
| 出版日期 | 2008-12-01 |
| 卷号 | 154期号:1-2页码:20-26 |
| 关键词 | West nile virus Monoclonal antibody Envelope protein domain iii Antigen capture-elisa |
| ISSN号 | 0166-0934 |
| DOI | 10.1016/j.jviromet.2008.09.019 |
| 通讯作者 | Gao, george f.(gaof@im.ac.cn) |
| 英文摘要 | Recent epidemics of west nile virus (wnv) around the world have been associated with significant rates of mortality and morbidity in humans. to develop standard wnv diagnostic tools that can differentiate wnv from japanese encephalitis virus (jev), four monoclonal antibodies (mabs) specific to wnv envelope (e) protein were produced and characterized by isotyping, reactivity with denatured and native antigens, affinity assay, immunofluorescence assay (ifa), and epitope competition. as well as cross-reactivity with jev. two of the mabs (6a11 and 4133) showed stronger reactivity with e protein than the others (2f5 and 6h7) in western blot analysis. 4133 could bind with denatured antigen, as well as native antigens in indirect elisa, flow cytometry analysis, and ifa: whereas 2f5 showed highest affinity with native antigen. 4133 and 2f5 were therefore used to establish an antigen capture-elisa(ac-elisa) detection system. the sensitivity of this ac-elisa was 3.95 tcid(50)/0.1 ml for wnv-infected cell culture supernatant. notably, these mabs showed no cross-reactivity with jev, which suggests that they are useful for further development of highly sensitive, easy handling, and less time-consuming detection kits/tools in wnv surveillance in areas where jev is epidemic. (c) 2008 elsevier b.v. all rights reserved. |
| WOS关键词 | LINKED IMMUNOSORBENT ASSAYS ; CAPTURE ENZYME-IMMUNOASSAY ; DOMAIN-III ; NUCLEOCAPSID PROTEIN ; ENCEPHALITIS ; ANTIGEN ; NEUTRALIZATION ; FLAVIVIRUSES ; IMMUNIZATION ; INFECTIONS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000261838800004 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2390465 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Gao, George F. |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Ctr Mol Immunol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, Ctr Mol Virol, Beijing 100101, Peoples R China 3.Acad Mil Med Sci, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China 4.China Anim Dis Control Ctr, Beijing 100094, Peoples R China 5.Nagasaki Univ, Inst Trop Med, Dept Virol, Nagasaki, Japan 6.Chinese Acad Sci, Inst Microbiol, China Japan Joint Lab Mol Immunol & Mol Microbiol, Beijing 100101, Peoples R China 7.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, June,Liu, Bohua,Cao, Zhen,et al. Characterization and application of monoclonal antibodies specific to west nile virus envelope protein[J]. Journal of virological methods,2008,154(1-2):20-26. |
| APA | Liu, June.,Liu, Bohua.,Cao, Zhen.,Inoue, Shingo.,Morita, Kouichi.,...&Gao, George F..(2008).Characterization and application of monoclonal antibodies specific to west nile virus envelope protein.Journal of virological methods,154(1-2),20-26. |
| MLA | Liu, June,et al."Characterization and application of monoclonal antibodies specific to west nile virus envelope protein".Journal of virological methods 154.1-2(2008):20-26. |
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来源：中国科学院大学
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