Protective effects of r-alpha-lipoic acid and acetyl-l-carnitine in min6 and isolated rat islet cells chronically exposed to oleic acid
文献类型：期刊论文
| 作者 | Shen, Weili2; Liu, Kai2; Tian, Chuan2,3; Yang, Lu2; Li, Xuesen2,3; Ren, Jinmin2; Packer, Lester4; Head, Elizabeth; Sharman, Edward; Liu, Jiankang1,2 |
| 刊名 | Journal of cellular biochemistry
 |
| 出版日期 | 2008-07-01 |
| 卷号 | 104期号:4页码:1232-1243 |
| 关键词 | Acetyl-l-carnitine Insulin secretion R-alpha-lipoic acid Mitochondria Oxidative stress Uncoupling protein (ucp)-2 Beta-cell |
| ISSN号 | 0730-2312 |
| DOI | 10.1002/jcb.21701 |
| 通讯作者 | Liu, jiankang(j.liu@uci.edu) |
| 英文摘要 | Mitochondrial dysfunction due to oxidative stress and concomitant impaired p-cell function may play a key role in type 2 diabetes. preventing and/or ameliorating oxidative mitochondrial dysfunction with mitochondria-specific nutrients may have preventive or therapeutic potential. in the present study, the oxidative mechanism of mitochondrial dysfunction in pancreatic beta-cells exposed to sublethal levels of oleic acid (oa) and the protective effects of mitochondrial nutrients [r-alpha-lipoic acid (la) and acetyl-l-carnitine (alc)] were investigated. chronic exposure (72 h) of insulinoma min6 cells to oa (0.2-0.8 mm) increased intracellular oxidant formation, decreased mitochondrial membrane potential (mmp), enhanced uncoupling protein-2 (ucp-2) mrna and protein expression, and consequently, decreased glucose-induced atp production and suppressed glucose-stimulated insulin secretion. pretreatment with la and/or alc reduced oxidant formation, increased mmp, regulated ucp-2 mrna and protein expression, increased glucose-induced atp production, and restored glucose-stimulated insulin secretion. the key findings on atp production and insulin secretion were verified with isolated rat islets. these results suggest that mitochondrial dysfunction is involved in oa-induced pancreatic p-cell dysfunction and that pretreatment with mitochondrial protective nutrients could be an effective strategy to prevent p-cell dysfunction. |
| WOS关键词 | PANCREATIC BETA-CELLS ; STIMULATED INSULIN-SECRETION ; FREE FATTY-ACIDS ; UNCOUPLING PROTEIN-2 ; MITOCHONDRIAL DYSFUNCTION ; INS-1 CELLS ; TRIGLYCERIDE ACCUMULATION ; METABOLIC ANTIOXIDANT ; OXIDATIVE DAMAGE ; HIGH GLUCOSE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000257567300010 |
| 出版者 | WILEY-LISS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2390983 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu, Jiankang |
| 作者单位 | 1.Univ Calif Irvine, Inst Brain Aging & Dementia, Gillespie Neurosci Res Facil 1261, Irvine, CA 92697 USA 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Shanghai 200031, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.Univ So Calif, Dept Mol Pharmacol & Pharmaceut Sci, Sch Pharm, Los Angeles, CA 90089 USA |
| 推荐引用方式 GB/T 7714 | Shen, Weili,Liu, Kai,Tian, Chuan,et al. Protective effects of r-alpha-lipoic acid and acetyl-l-carnitine in min6 and isolated rat islet cells chronically exposed to oleic acid[J]. Journal of cellular biochemistry,2008,104(4):1232-1243. |
| APA | Shen, Weili.,Liu, Kai.,Tian, Chuan.,Yang, Lu.,Li, Xuesen.,...&Liu, Jiankang.(2008).Protective effects of r-alpha-lipoic acid and acetyl-l-carnitine in min6 and isolated rat islet cells chronically exposed to oleic acid.Journal of cellular biochemistry,104(4),1232-1243. |
| MLA | Shen, Weili,et al."Protective effects of r-alpha-lipoic acid and acetyl-l-carnitine in min6 and isolated rat islet cells chronically exposed to oleic acid".Journal of cellular biochemistry 104.4(2008):1232-1243. |
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来源：中国科学院大学
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