Characterization of the novel gene bjdreb1b encoding a dre-binding transcription factor from brassica juncea l.
文献类型：期刊论文
| 作者 | Cong, Lin1; Chai, Tuan-Yao1; Zhang, Yu-Xiu2 |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2008-07-11 |
| 卷号 | 371期号:4页码:702-706 |
| 关键词 | Brassica juncea l. Dre-binding protein Transcription factor Drought stress Salt stress |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2008.04.126 |
| 通讯作者 | Chai, tuan-yao(tychai@gucas.ac.cn) |
| 英文摘要 | A novel dreb (dehydration responsive element binding protein) gene, designated bjdreb1b, was isolated from brassica juncea l. bjdreb1b contains a conserved erebp/ap2 domain and was classified into the a-1 subgroup of the dreb subfamily based on phylogenetic tree analysis. rt-pcr showed that bjdreb1b was induced by abiotic stresses and exogenous phytohormones, such as drought, salt, low temperature, heavy metals, abscisic acid, and salicylic acid. gel shift assay revealed that bjdreb1b specifically bound to the dre element in vitro. yeast one-hybrid assay showed that full-length bjdreb1b or its c-terminal region functioned effectively as a trans-activator. furthermore, overexpression of bjdreb1b in tobacco up-regulated the expression of nterd10b, and bjdreb1b transgenic plants accumulated higher levels of proline than control plants under normal and saline conditions, together showing that bjdreb1b plays important roles in improving plant tolerance to drought and salinity. (c) 2008 elsevier inc. all rights reserved. |
| WOS关键词 | LOW-TEMPERATURE ; SALICYLIC-ACID ; HIGH-SALT ; RESPONSIVE GENES ; OSMOTIC-STRESS ; ARABIDOPSIS ; DROUGHT ; EXPRESSION ; TOLERANCE ; DEHYDRATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000256506600025 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2390993 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chai, Tuan-Yao |
| 作者单位 | 1.Chinese Acad Sci, Grad Univ, Coll Life Sci, Beijing 100049, Peoples R China 2.China Univ Min & Technol, Sch Chem & Environm Engn, Dept Bioengn, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cong, Lin,Chai, Tuan-Yao,Zhang, Yu-Xiu. Characterization of the novel gene bjdreb1b encoding a dre-binding transcription factor from brassica juncea l.[J]. Biochemical and biophysical research communications,2008,371(4):702-706. |
| APA | Cong, Lin,Chai, Tuan-Yao,&Zhang, Yu-Xiu.(2008).Characterization of the novel gene bjdreb1b encoding a dre-binding transcription factor from brassica juncea l..Biochemical and biophysical research communications,371(4),702-706. |
| MLA | Cong, Lin,et al."Characterization of the novel gene bjdreb1b encoding a dre-binding transcription factor from brassica juncea l.".Biochemical and biophysical research communications 371.4(2008):702-706. |
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来源：中国科学院大学
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