Identification of the orotidine-5 '-monophosphate decarboxylase gene of the oleaginous yeast rhodosporidium toruloides
文献类型：期刊论文
| 作者 | Yang, Fan1,2; Zhang, Sufang1; Tang, Wei1,2; Zhao, Zongbao K.1 |
| 刊名 | Yeast
 |
| 出版日期 | 2008-09-01 |
| 卷号 | 25期号:9页码:623-630 |
| ISSN号 | 0749-503X |
| 关键词 | Rhodosporidium tortiloides Lipids Orotidine 5 '-monophosphate decarboxylase Restriction-free cloning Complementation |
| DOI | 10.1002/yea.1607 |
| 通讯作者 | Zhao, zongbao k.(zhoozb@dicp.ac.cn) |
| 英文摘要 | Oleaginous yeast rhodosporidium toraloides is an excellent microbial lipid producer of great industrial potential, yet there is no effective genetic tool for rationally engineering this microorganism. to develop a marker recycling system, the orotidine-5 '-monophosphate (omp) decarboxylase gene of r. toruloides (rtura3) was isolated using methods of degenerate pollymerase chain reaction (pcr) together with rapid amplification of cdna ends. the results showed that rtura3 contains four extrons and three introns, and that the encoded polypeptide holds a sequence of 279 amino acid residues with significant homology to those of omp decarboxylases from other yeasts. a shuttle vector pyes2/ct-rtura3 was constructed via site-specific insertion of rtura3 into the commercial vector pyes2/ct. transformation of the shuttle vector into saccharomyces cerevisiae by4741, a ura3-deficient yeast strain, ensured the viability of the strain on synthetic dextrose agar plate without uracil, suggesting that the isolated rtura3 was functionally equivalent to the ura3 gene from s. cerevisiae. the genbank accession nos for the cdna and genomic dna sequences of rtura3 are eu693528 and eu693529, respectively. copyright (c) 2008 john wiley & sons, ltd. |
| WOS关键词 | LIPID-ACCUMULATION ; TRANSFORMATION ; SELECTION ; PLASMIDS ; CULTURE ; URA3 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology |
| 语种 | 英语 |
| 出版者 | JOHN WILEY & SONS LTD |
| WOS记录号 | WOS:000259537700002 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2390997 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhao, Zongbao K. |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Fan,Zhang, Sufang,Tang, Wei,et al. Identification of the orotidine-5 '-monophosphate decarboxylase gene of the oleaginous yeast rhodosporidium toruloides[J]. Yeast,2008,25(9):623-630. |
| APA | Yang, Fan,Zhang, Sufang,Tang, Wei,&Zhao, Zongbao K..(2008).Identification of the orotidine-5 '-monophosphate decarboxylase gene of the oleaginous yeast rhodosporidium toruloides.Yeast,25(9),623-630. |
| MLA | Yang, Fan,et al."Identification of the orotidine-5 '-monophosphate decarboxylase gene of the oleaginous yeast rhodosporidium toruloides".Yeast 25.9(2008):623-630. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。