Preparation of colloidal gold immunochromatography strip for detection of methamidophos residue
文献类型：期刊论文
| 作者 | Shi Chenggang2; Zhao Suqing1,3,5; Zhang Kun1; Hong Guobao2; Zhu Zhenyu4 |
| 刊名 | Journal of environmental sciences-china
 |
| 出版日期 | 2008 |
| 卷号 | 20期号:11页码:1392-1397 |
| 关键词 | Colloidal gold Immunochromatography Methamidophos (met) |
| ISSN号 | 1001-0742 |
| 通讯作者 | Zhao suqing(suqingzhao@yahoo.com.cn) |
| 英文摘要 | Methamidophos (met) is a broad spectrum organophosphorus insecticide and acaricide. even a trace of its residue is harmful to humans and many animals. in this study, the synthesis and identification of colloidal gold particles and antibody-colloidal gold conjugates were performed, and the preparation of colloidal gold immunochromatography strip was conducted for detection of met residue. the size of colloidal gold particles was checked using a transmission electron microscope (tem). the formation of antibody-colloidal gold conjugates was monitored by uv/vis spectroscopy. the preparation of colloidal gold immunochromatography strips, analysis of met standard solutions, and other four pesticides and vegetable samples were operated with common methods and principals. the tem images showed the average diameter of colloidal gold particles was almost the same size: approximately 40.0 nm in diameter. for the conjugation of colloidal gold and monoclonal antibody (mab), 0.03 mg/ml of mab was confirmed to be the minimum amount for stabilization of colloidal gold. with the prepared colloidal gold immunochromatography test strip to determine the standard met solution, the results demonstrated a detection limit of approximately 1.0 [mu g/ml. cross-reaction indicated that the strip had a high specificity to met. the results of 10 green vegetable sample tests confirmed that one sample was positive by hplc analysis. there was evidence to suggest that colloidal gold particles and anti body-colloidal gold conjugates were synthesized successfully. the prepared colloidal gold immunochromatography strip was applicable for preliminary screening of met residue. |
| WOS关键词 | ANTIBODY PROBE ; GREENHOUSE ; TOMATOES ; ASSAY |
| WOS研究方向 | Environmental Sciences & Ecology |
| WOS类目 | Environmental Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000260879500017 |
| 出版者 | SCIENCE PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2391185 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhao Suqing |
| 作者单位 | 1.Guangdong Univ Technol, Fac Chem Engn & Light Ind, Dept Pharm Engn, Guangzhou 510006, Peoples R China 2.Sun Yat Sen Univ Med Sci, Affiliated Hosp 3, Guangzhou 510630, Peoples R China 3.Guangxi Inst Bot, Guilin 541006, Peoples R China 4.Sun Yat Sen Univ, Zhongshan Med Coll, Dept Biochem, Guangzhou 510080, Peoples R China 5.Acad Sinica, Guilin 541006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi Chenggang,Zhao Suqing,Zhang Kun,et al. Preparation of colloidal gold immunochromatography strip for detection of methamidophos residue[J]. Journal of environmental sciences-china,2008,20(11):1392-1397. |
| APA | Shi Chenggang,Zhao Suqing,Zhang Kun,Hong Guobao,&Zhu Zhenyu.(2008).Preparation of colloidal gold immunochromatography strip for detection of methamidophos residue.Journal of environmental sciences-china,20(11),1392-1397. |
| MLA | Shi Chenggang,et al."Preparation of colloidal gold immunochromatography strip for detection of methamidophos residue".Journal of environmental sciences-china 20.11(2008):1392-1397. |
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来源：中国科学院大学
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