Generation and characterization of transgenic poplar plants overexpressing a cotton laccase gene
文献类型：期刊论文
| 作者 | Wang, Ji1,2; Wang, Chenglong1,2; Zhu, Mulan1,2; Yu, Yang1,2; Zhang, Yuebo3; Wei, Zhiming1 |
| 刊名 | Plant cell tissue and organ culture
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 93期号:3页码:303-310 |
| 关键词 | Laccase Lignin Phenolic metabolism Populus Transgenic poplars |
| ISSN号 | 0167-6857 |
| DOI | 10.1007/s11240-008-9377-x |
| 通讯作者 | Wei, zhiming(zmwei@sippe.ac.cn) |
| 英文摘要 | Laccases are copper-containing glycoproteins, which are widespread in higher plants as multigene families. to gain more insight in the function of laccases in plants, especially potential role in lignification, we produced transgenic poplar plants overexpressing a cotton laccase cdna (galac1) under the control of the cauliflower mosaic virus 35s promoter. as compared with untransformed control plants, transgenic plants exhibited a 2.1- to 13.2-fold increased laccase activity, whereas plant growth rate and morphological characters remained similar to control plants. a 2.1-19.6% increase in total lignin content of the stem was found in transgenic plants. moreover, transgenic plants showed a dramatically accelerated oxidation rate of phenolics, without obvious change in total phenolic content. our data suggested that galac1 may participate in lignin synthesis and phenolic metabolism in plants. the present work provided a new genetic evidence for the involvement of plant laccases in lignification. |
| WOS关键词 | POLYPHENOL OXIDASE ; LIGNIN ; ARABIDOPSIS ; RESISTANCE ; ENZYME ; PEROXIDASE ; EXPRESSION ; DEPOSITION ; STRESS ; GENOME |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Plant Sciences |
| WOS类目 | Biotechnology & Applied Microbiology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000255987000007 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2391353 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wei, Zhiming |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutrit Sci, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ji,Wang, Chenglong,Zhu, Mulan,et al. Generation and characterization of transgenic poplar plants overexpressing a cotton laccase gene[J]. Plant cell tissue and organ culture,2008,93(3):303-310. |
| APA | Wang, Ji,Wang, Chenglong,Zhu, Mulan,Yu, Yang,Zhang, Yuebo,&Wei, Zhiming.(2008).Generation and characterization of transgenic poplar plants overexpressing a cotton laccase gene.Plant cell tissue and organ culture,93(3),303-310. |
| MLA | Wang, Ji,et al."Generation and characterization of transgenic poplar plants overexpressing a cotton laccase gene".Plant cell tissue and organ culture 93.3(2008):303-310. |
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来源：中国科学院大学
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