Genome-scale mutagenesis and phenotypic characterization of two-component signal transduction systems in xanthomonas campestris pv. campestris atcc 33913
文献类型：期刊论文
| 作者 | Qian, Wei1,2; Han, Zhong-Ji1; Tao, Jun1,3; He, Chaozu1 |
| 刊名 | Molecular plant-microbe interactions
 |
| 出版日期 | 2008-08-01 |
| 卷号 | 21期号:8页码:1128-1138 |
| 关键词 | Reverse-genetic approach |
| ISSN号 | 0894-0282 |
| DOI | 10.1094/mpmi-21-8-1128 |
| 通讯作者 | He, chaozu(hecz@sun.im.ac.cn) |
| 英文摘要 | The gram-negative bacterium xanthomonas campestris pv. campestris is the causal agent of black rot disease of cruciferous plants. its genome encodes a large repertoire of two-component signal transduction systems (tcstss), which consist of histidine kinases and response regulators (rr) to monitor and respond to environmental stimuli. to investigate the biological functions of these tcsts genes, we aimed to inactivate all 54 rr genes in x. campestris pv. campestris atcc 33913, and successfully generated 51 viable mutants using the insertion inactivation method. plant inoculation identified two novel response regulator genes (xcc1958 and xcc3107) that are involved in virulence of this strain. genetic complementation demonstrated that xcc3107, designated as vgrr (virulence and growth regulator), also affects bacterial growth and activity of extracellular proteases. in addition, we assessed the survival of these mutants under various stresses, including osmotic stress, high sodium concentration, heat shock, and sodium dodecyl sulfate exposure, and identified a number of genes that may be involved in the general stress response of x. campestris pv. campestris. mutagenesis and phenotypic characterization of rr genes in this study will facilitate future studies on signaling networks in this important phytopathogenic bacterium. |
| WOS关键词 | CYCLIC-DI-GMP ; GYP DOMAIN PROTEIN ; ESCHERICHIA-COLI ; PSEUDOMONAS-AERUGINOSA ; MYCOBACTERIUM-TUBERCULOSIS ; MOLECULAR CHARACTERIZATION ; ENTEROCOCCUS-FAECALIS ; PATHOVAR CAMPESTRIS ; RESPONSE REGULATOR ; BACILLUS-SUBTILIS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000257728200012 |
| 出版者 | AMER PHYTOPATHOLOGICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2391523 |
| 专题 | 中国科学院大学 |
| 通讯作者 | He, Chaozu |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Gen, Beijing 100101, Peoples R China 2.Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qian, Wei,Han, Zhong-Ji,Tao, Jun,et al. Genome-scale mutagenesis and phenotypic characterization of two-component signal transduction systems in xanthomonas campestris pv. campestris atcc 33913[J]. Molecular plant-microbe interactions,2008,21(8):1128-1138. |
| APA | Qian, Wei,Han, Zhong-Ji,Tao, Jun,&He, Chaozu.(2008).Genome-scale mutagenesis and phenotypic characterization of two-component signal transduction systems in xanthomonas campestris pv. campestris atcc 33913.Molecular plant-microbe interactions,21(8),1128-1138. |
| MLA | Qian, Wei,et al."Genome-scale mutagenesis and phenotypic characterization of two-component signal transduction systems in xanthomonas campestris pv. campestris atcc 33913".Molecular plant-microbe interactions 21.8(2008):1128-1138. |
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来源：中国科学院大学
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