Characterization of two glutaminases from the filamentous cyanobacterium anabaena sp pcc 7120
文献类型：期刊论文
| 作者 | Zhou, Jun Xia1,2; Zhou, Jie1; Yang, Hao Meng1; Chen, Mei1,2; Huang, Fang1 |
| 刊名 | Fems microbiology letters
 |
| 出版日期 | 2008-12-01 |
| 卷号 | 289期号:2页码:241-249 |
| 关键词 | Anabaena sp Pcc 7120 Recombinant glutaminase Phosphate-activated Nitrogen depletion |
| ISSN号 | 0378-1097 |
| DOI | 10.1111/j.1574-6968.2008.01395.x |
| 通讯作者 | Huang, fang(fhuang@ibcas.ac.cn) |
| 英文摘要 | The anabaena genome contains two orfs that appear to encode glutaminases. the genes were expressed as histidine-tagged fusion proteins in escherichia coli. the purified proteins possessed glutaminase activity using l-glutamine as the substrate, but differed in biochemical properties. all2934 showed an optimal activity at 20 degrees c and ph 6.0, with a higher affinity for l-glutamine than all4774, which had optimal activity at 37 degrees c and ph 7.5. remarkably, the glutaminase activity of all2934 was phosphate dependent, while all4774 was phosphate independent. the expression of all2934 and all4774 was analyzed using semi-quantitative reverse transcriptase-pcr. the expression level of all2934 was much higher than that of all4774 under normal and nitrogen-depletion conditions, indicating that all2934 may play an important role in metabolizing glutamine in anabaena. |
| WOS关键词 | SP-STRAIN PCC-7120 ; PHOSPHATE-ACTIVATED GLUTAMINASE ; DEPENDENT GLUTAMINASE ; PURIFICATION ; HETEROCYSTS ; ASPARAGINASE ; CYLINDRICA ; METABOLISM ; EXPRESSION ; GLUTAMATE |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000261123200017 |
| 出版者 | WILEY-BLACKWELL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2391699 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Huang, Fang |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynthesis & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Jun Xia,Zhou, Jie,Yang, Hao Meng,et al. Characterization of two glutaminases from the filamentous cyanobacterium anabaena sp pcc 7120[J]. Fems microbiology letters,2008,289(2):241-249. |
| APA | Zhou, Jun Xia,Zhou, Jie,Yang, Hao Meng,Chen, Mei,&Huang, Fang.(2008).Characterization of two glutaminases from the filamentous cyanobacterium anabaena sp pcc 7120.Fems microbiology letters,289(2),241-249. |
| MLA | Zhou, Jun Xia,et al."Characterization of two glutaminases from the filamentous cyanobacterium anabaena sp pcc 7120".Fems microbiology letters 289.2(2008):241-249. |
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来源：中国科学院大学
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