Cloning, expression, and characterization of two novel cuticle-degrading serine proteases from the entomopathogenic, fungus cordyceps sinensis
文献类型：期刊论文
| 作者 | Zhang, Yongjie1,2; Liu, Xingzhong1; Wang, Mu3 |
| 刊名 | Research in microbiology
 |
| 出版日期 | 2008-07-01 |
| 卷号 | 159期号:6页码:462-469 |
| 关键词 | Cordyceps sinensis Serine protease Entomopathogenic fungus Pichia pastoris Characterization Traditional chinese medicine |
| ISSN号 | 0923-2508 |
| DOI | 10.1016/j.resmic.2008.04.004 |
| 通讯作者 | Liu, xingzhong(liuxz@sun.im.ac.cn) |
| 英文摘要 | The entomopathogenic fungus cordyceps sinensis has been important in traditional chinese medicine, but is yet to be commercially cultivated. difficulty in cultivation results in part from the low percentage of fungal infection on artificially inoculated host insects. to better understand the infection mechanism, we cloned two cuticle-degrading serine protease genes (csp1 and csp2) from c. sinensis. these enzymes are novel members of the s8a subfamily of proteases. identities of cdna or amino acid sequences between cspi and csp2 were 72.9% and 68.9%, respectively. after successful expression in the yeast pichia pastoris, recombinant enzymes were purified and characterized using the synthetic substrate n-suc-aapf-p-na. both were chymotrypsin-like serine proteases with an optimum ph of 7.0 and an optimal temperature of 40 degrees c (cspl) or 50 degrees c (csp2). bioassay revealed that cspi and csp2 degraded the cuticle proteins of larval hepialus sp. in vitro. this is the first report of serine proteases from c. sinensis. (c) 2008 elsevier masson sas. all rights reserved. |
| WOS关键词 | TRADITIONAL CHINESE MEDICINE ; METARHIZIUM-ANISOPLIAE ; INSECT CUTICLE ; SCIENTIFIC REDISCOVERY ; MANDUCA-SEXTA ; VIRULENCE ; CULTURE ; ENZYMES ; GENE |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000259829200008 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2391705 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu, Xingzhong |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Key Lab Systemat Mycol & Lichenol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Agr & Anim Husb Coll Tibet, Linzhi 860000, Tibet, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yongjie,Liu, Xingzhong,Wang, Mu. Cloning, expression, and characterization of two novel cuticle-degrading serine proteases from the entomopathogenic, fungus cordyceps sinensis[J]. Research in microbiology,2008,159(6):462-469. |
| APA | Zhang, Yongjie,Liu, Xingzhong,&Wang, Mu.(2008).Cloning, expression, and characterization of two novel cuticle-degrading serine proteases from the entomopathogenic, fungus cordyceps sinensis.Research in microbiology,159(6),462-469. |
| MLA | Zhang, Yongjie,et al."Cloning, expression, and characterization of two novel cuticle-degrading serine proteases from the entomopathogenic, fungus cordyceps sinensis".Research in microbiology 159.6(2008):462-469. |
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来源：中国科学院大学
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