In vitro multiplication of heavy metals hyperaccumulator thlaspi caerulescens
文献类型:期刊论文
作者 | Xu, J.1; Zhang, Y. X.2; Chai, T. Y.1; Guan, Z. Q.1; Wei, W.1; Han, L.1; Cong, L.1 |
刊名 | Biologia plantarum
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出版日期 | 2008-03-01 |
卷号 | 52期号:1页码:97-100 |
关键词 | Micropropagation Growth regulators Riboflavin |
ISSN号 | 0006-3134 |
DOI | 10.1007/s10535-008-0016-5 |
通讯作者 | Chai, t. y.(tychai@gucas.ac.cn) |
英文摘要 | A micropropagation protocol through multiple shoot formation was developed for thlaspi caerulescens l., one of the most important heavy metals hyperaccumulator plants. in vitro seed-derived young seedlings were used for the initiation of multiple shoots on murashige and skoog (ms) medium with combinations of benzylaminopurine (ba; 0.5-1.0 mg dm(-3)), naphthaleneacetic acid (naa; 0-0.2 mg dm(-3)), gibberellic acid (ga(3); 0-1.0 mg dm(-3)) and riboflavin (0-3.0 mg dm(-3)). the maximum number of shoots was developed on medium containing 1.0 mg dm(-3) ba and 0.2 mg dm(-3) naa. ga(3) (0.5 mg dm(-3)) in combination with ba significantly increased shoot length. in view of shoot numbers, shoot length and further rooting rate, the best combination was 1.0 mg dm(-3) ba + 0.5 mg dm(-3) ga(3) + 1.0 mg dm(-3) riboflavin. well-developed shoots (35-50 mm) were successfully rooted at approximately 95 % on ms medium containing 20 g dm-3 sucrose, 8 g dm(-3) agar and 1.0 mg dm(-3) indolebutyric acid. almost all in vitro plantlets survived when transferred to pots. |
WOS研究方向 | Plant Sciences |
WOS类目 | Plant Sciences |
语种 | 英语 |
WOS记录号 | WOS:000253754600014 |
出版者 | SPRINGER |
URI标识 | http://www.irgrid.ac.cn/handle/1471x/2392181 |
专题 | 中国科学院大学 |
通讯作者 | Chai, T. Y. |
作者单位 | 1.Grad Univ, Chinese Acad Sci, Dept Biol, Beijing 100049, Peoples R China 2.China Univ Min & Technol, Dept Bioengn, Beijing 100083, Peoples R China |
推荐引用方式 GB/T 7714 | Xu, J.,Zhang, Y. X.,Chai, T. Y.,et al. In vitro multiplication of heavy metals hyperaccumulator thlaspi caerulescens[J]. Biologia plantarum,2008,52(1):97-100. |
APA | Xu, J..,Zhang, Y. X..,Chai, T. Y..,Guan, Z. Q..,Wei, W..,...&Cong, L..(2008).In vitro multiplication of heavy metals hyperaccumulator thlaspi caerulescens.Biologia plantarum,52(1),97-100. |
MLA | Xu, J.,et al."In vitro multiplication of heavy metals hyperaccumulator thlaspi caerulescens".Biologia plantarum 52.1(2008):97-100. |
入库方式: iSwitch采集
来源:中国科学院大学
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