Redundant requirement for a pair of protein arginine methyltransferase4 homologs for the proper regulation of arabidopsis flowering time
文献类型：期刊论文
| 作者 | Niu, Lifang1,2,3; Zhang, Yong1,2,3; Pei, Yanxi1,2,4; Liu, Chunyan1,2; Cao, Xiaofeng1,2 |
| 刊名 | Plant physiology
 |
| 出版日期 | 2008-09-01 |
| 卷号 | 148期号:1页码:490-503 |
| ISSN号 | 0032-0889 |
| DOI | 10.1104/pp.108.124727 |
| 通讯作者 | Cao, xiaofeng(xfcao@genetics.ac.cn) |
| 英文摘要 | Carm1/prmt4 (for coactivator-associated arginine methyltransferase1/protein arginine methyltransferase4) catalyzes asymmetric dimethylation on arginine (arg), and its functions in gene regulation is understood only in animal systems. here, we describe atprmt4a and atprmt4b as a pair of arabidopsis (arabidopsis thaliana) homologs of mammalian carm1/prmt4. recombinant atprmt4a and atprmt4b could asymmetrically dimethylate histone h3 at arg-2, arg-17, arg-26, and myelin basic protein in vitro. both atprmt4a and atprmt4b exhibited nuclear as well as cytoplasmic distribution and were expressed ubiquitously in all tissues throughout development. glutathione s-transferase pull-down assays revealed that atprmt4a and atprmt4b could form homodimers and heterodimers in vitro, and formation of the heterodimer was further confirmed by bimolecular fluorescence complementation. simultaneous lesions in atprmt4a and atprmt4b genes led to delayed flowering, whereas single mutations in either atprmt4a or atprmt4b did not cause major developmental defects, indicating the redundancy of atprmt4a and atprmt4b. genetic analysis also indicated that atprmt4a atprmt4b double mutants phenocopied autonomous pathway mutants. finally, we found that asymmetric methylation at arg-17 of histone h3 was greatly reduced in atprmt4a atprmt4b double mutants. taken together, our results demonstrate that atprmt4a and atprmt4b are required for proper regulation of flowering time mainly through the flowering locus c-dependent pathway. |
| WOS关键词 | ARGININE METHYLTRANSFERASE CARM1 ; HISTONE MODIFICATION PATTERNS ; RNA-BINDING PROTEIN ; MADS-BOX GENE ; LOCUS-C ; TRANSCRIPTIONAL ACTIVATION ; RECEPTOR FUNCTION ; COACTIVATOR COMPLEX ; FLORAL TRANSITION ; HORMONE-RECEPTOR |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000258947600042 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2392406 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Cao, Xiaofeng |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Shanxi Univ, Coll Life Sci & Technol, Taiyuan 030006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Niu, Lifang,Zhang, Yong,Pei, Yanxi,et al. Redundant requirement for a pair of protein arginine methyltransferase4 homologs for the proper regulation of arabidopsis flowering time[J]. Plant physiology,2008,148(1):490-503. |
| APA | Niu, Lifang,Zhang, Yong,Pei, Yanxi,Liu, Chunyan,&Cao, Xiaofeng.(2008).Redundant requirement for a pair of protein arginine methyltransferase4 homologs for the proper regulation of arabidopsis flowering time.Plant physiology,148(1),490-503. |
| MLA | Niu, Lifang,et al."Redundant requirement for a pair of protein arginine methyltransferase4 homologs for the proper regulation of arabidopsis flowering time".Plant physiology 148.1(2008):490-503. |
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来源：中国科学院大学
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