Dissection of the regulatory mechanism of a heat-shock responsive promoter in haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation
文献类型：期刊论文
| 作者 | Lu, Qiuhe1,2; Han, Jing1,2; Zhou, Ligang1,2; Coker, James A.3; DasSarma, Priya3; DasSarma, Shiladitya3; Xiang, Hua1 |
| 刊名 | Nucleic acids research
 |
| 出版日期 | 2008-05-01 |
| 卷号 | 36期号:9页码:3031-3042 |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gkn152 |
| 通讯作者 | Xiang, hua(xiangh@sun.im.ac.cn) |
| 英文摘要 | Multiple general transcription factors (gtfs), tbp and tfb, are present in many haloarchaea, and are deemed to accomplish global gene regulation. however, details and the role of gtf-directed transcriptional regulation in stress response are still not clear. here, we report a comprehensive investigation of the regulatory mechanism of a heat-induced gene (hsp5) from halobacterium salinarum. we demonstrated by mutation analysis that the sequences 5' and 3' to the core elements (tata box and bre) of the hsp5 promoter (p(hsp5)) did not significantly affect the basal and heat-induced gene expression, as long as the transcription initiation site was not altered. moreover, the bre and tata box of phsp5 were sufficient to render a nonheat-responsive promoter heat-inducible, in both haloferax volcanii and halobacterium sp. nrc1. dna-protein interactions revealed that two heat-inducible gtfs, tfb2 from h. volcanii and tfbb from halobacterium sp. nrc-1, could specifically bind to phsp5 likely in a temperature-dependent manner. taken together, the heat-responsiveness of phsp5 was mainly ascribed to the core promoter elements that were efficiently recognized by specific heat- induced gtfs at elevated temperature, thus providing a new paradigm for gtf-directed gene regulation in the domain of archaea. |
| WOS关键词 | PYROCOCCUS-FURIOSUS ; HALOFERAX-VOLCANII ; GENOME SEQUENCE ; HALOBACTERIUM-SALINARUM ; STREPTOMYCES-ALBUS ; HALOPHILIC ARCHAEA ; ALPHA-CRYSTALLIN ; REPORTER GENE ; EXPRESSION ; PROTEINS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000256173200028 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2392481 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xiang, Hua |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Univ Maryland, Inst Biotechnol, Ctr Marine Biotechnol, Baltimore, MD 21202 USA |
| 推荐引用方式 GB/T 7714 | Lu, Qiuhe,Han, Jing,Zhou, Ligang,et al. Dissection of the regulatory mechanism of a heat-shock responsive promoter in haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation[J]. Nucleic acids research,2008,36(9):3031-3042. |
| APA | Lu, Qiuhe.,Han, Jing.,Zhou, Ligang.,Coker, James A..,DasSarma, Priya.,...&Xiang, Hua.(2008).Dissection of the regulatory mechanism of a heat-shock responsive promoter in haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation.Nucleic acids research,36(9),3031-3042. |
| MLA | Lu, Qiuhe,et al."Dissection of the regulatory mechanism of a heat-shock responsive promoter in haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation".Nucleic acids research 36.9(2008):3031-3042. |
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来源：中国科学院大学
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